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通过注射和电穿孔的斑马鱼Otocyst的MIF Morpholinos直接运抵影响内耳发展
JoVE 신문
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JoVE 신문 생물학
Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
DOI:

10:40 min

January 07, 2011

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Chapters

  • 00:05Title
  • 01:22Introduction
  • 02:45Making Electrodes and Set Up of Electroporation Station
  • 04:09Microinjection of Morpholinos into the Zebrafish Otic Vesicle
  • 06:52Electroporation Procedure
  • 08:13Representative Ear Injection Results
  • 10:21Conclusion

Summary

자동 번역

在24hpf的斑马鱼otocyst morpholinos直接传递到一个方法来已经研制成功。管腔奥迪的囊泡和电的影响渗透到使用morpholinos显微注射,我们能够绕过morpholinos对大脑的影响,并获得具体到内耳的影响。

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