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通过注射和电穿孔的斑马鱼Otocyst的MIF Morpholinos直接运抵影响内耳发展
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January 07, 2011
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1Department of Veterinary Science,University of Wisconsin, Madison, 2Department of Cell and Developmental Biology,University of Michigan, Ann Arbor, MI, 3Present address: Department of Pulmonary Medicine,University of Michigan, Ann Arbor, MI, 4Department of Biomedical Engineering,University of Michigan, Ann Arbor, MI
Summary
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在24hpf的斑马鱼otocyst morpholinos直接传递到一个方法来已经研制成功。管腔奥迪的囊泡和电的影响渗透到使用morpholinos显微注射，我们能够绕过morpholinos对大脑的影响，并获得具体到内耳的影响。
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Holmes, K. E., Wyatt, M. J., Shen, Y., Thompson, D. A., Barald, K. F. Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development. J. Vis. Exp. (47), e2466, doi:10.3791/2466 (2011).
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