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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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13:49 min
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January 11, 2011
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In many biological and clinical situations it is advantageous to study cellular processes as they evolve in their native microenvironment. Here we describe the assembly and use of a low-cost fiber-optic microscope which can provide real time imaging in cell culture, animal studies, and clinical patient studies.
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High-resolution Fiber-optic MicroendoscopyIn Situ Cellular ImagingLongitudinal StudyMorphology And FunctionLight MicroscopyIntravital TechniquesConfocal MicroscopyMultiphoton MicroscopySecond Harmonic MicroscopyHigh-resolution MicroendoscopeOff-the-shelf ComponentsImage ResolutionField-of-viewOperating WavelengthEnd UserIn Vitro Cell CultureFluorescent Contrast AgentsProflavineBenzoporphyrin-derivative Monoacid Ring A (BPD-MA)Fluoroscein
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Pierce, M., Yu, D., Richards-Kortum, R. High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging. J. Vis. Exp. (47), e2306, doi:10.3791/2306 (2011).
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