JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
A Method for Generating Oligodendrocyte-Conditioned Medium
내레이션 대본
Plate two or three pre-coated 100-millimeter Petri dishes with 10 milliliters of the cell suspension.
Incubate in a humidified incubator at 37 degrees Celsius under 5% carbon dioxide.
In a laminar flow hood under sterile conditions, renew the culture medium with 10 milliliters of warm Mbb 27 low medium.
Incubate for 2 days in a humidified incubator at 37 degrees Celsius under 5% carbon dioxide.
To harvest the OCM, collect the supernatant containing oligodendrocyte-secreted factors. Filter-sterilize the OCM using a 0.22-micron filter.
