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Cryopreserving e recupero di cellule umane iPS usando KnockOut Completa alimentatore-Free siero sostituzione Media
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July 15, 2010
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Questo protocollo descrive la procedura dettagliata per cryopreserving cellule umane iPS nel medio KnockOut crioconservazione SR ed il recupero di queste cellule in completo alimentatore KnockOut gratuito SR (KSR-FF), medio o alimentatore a base di medie SR KnockOut.
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CryopreservingRecoveringHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-free MediumReprogrammingFibroblastsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementEmbryonic Stem Cell GrowthIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Or Human Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated Cells
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Wagner, K., Welch, D. Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium. J. Vis. Exp. (41), e2237, doi:10.3791/2237 (2010).
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