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Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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July 15, 2010
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This protocol describes the detailed procedure for cryopreserving human iPS cells in KnockOut SR cryopreservation medium and recovering these cells in complete KnockOut SR Feeder Free (KSR-FF) medium or feeder-based KnockOut SR medium.
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CryopreservingRecoveringHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-free MediumReprogrammingFibroblastsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementEmbryonic Stem Cell GrowthIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Or Human Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated Cells
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Wagner, K., Welch, D. Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium. J. Vis. Exp. (41), e2237, doi:10.3791/2237 (2010).
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