Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models
Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models
내레이션 대본
Obtain a sterile intubation device consisting of a metal cannula with polyethylene tubing connected to a metal needle.
Attach a syringe containing Streptococcuspneumoniae to the needle, and fill the tubing.
Next, position an anesthetized immunocompetent rat in a supine position.
Insert the free end of the metal cannula into the rat's mouth. Advance it into the trachea toward the left bronchus and down the left lung to an appropriate depth.
Advance the tubing to the end of the cannula and instill the bacterial suspension into the left lung lobe.
The bacterial capsule and surface-associated enzymes prevent bacterial entrapment in the mucus. Further, Streptococcuspneumoniae attach to the surface receptors on bronchial epithelial cells, facilitating endocytosis and release to the basolateral surface.
Bacteria secrete toxins that damage the epithelial cells and barriers, triggering an immune response.
However, Streptococcuspneumoniae has developed strategies to evade the host immune response and establish lung infection.