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Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
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October 03, 2010
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This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.
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Intracranial Orthotopic AllograftingMedulloblastoma CellsImmunocompromised MicePediatric TumorNervous SystemCerebellar Granule Neuron PrecursorsCGNPsCell-of-originClinical PresentationsMedulloblastoma SubtypesCellular And Molecular OriginsEctopic ExpressionCGNP MarkerTumor-initiating CellsExperimental ProtocolEstablished Cell Lines
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Huang, X., Sarangi, A., Ketova, T., Litingtung, Y., Cooper, M. K., Chiang, C. Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice. J. Vis. Exp. (44), e2153, doi:10.3791/2153 (2010).
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