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Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
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Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
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August 13, 2010
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InductionAssessmentClass Switch RecombinationPurifiedMurine B CellsHumoral ImmunityB Cell ActivationImmunoglobulin LocusGenetic ModificationsAntibodiesIgM AntibodyIgGIgAIgEEffector FunctionsImmunity MaintenanceDiversificationImmunoglobulin LocusActivation-induced Cytidine Deaminase (AID)CSR PathwayB Cell FunctionMouse ModelSpleen IsolationBacterial Lipopolysaccharide (LPS)IgG3 SwitchingFluorescent Cell Staining Dye CFSE
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Zaheen, A., Martin, A. Induction and Assessment of Class Switch Recombination in Purified Murine B Cells. J. Vis. Exp. (42), e2130, doi:10.3791/2130 (2010).
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