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Generation of Recombinant Influenza Virus from Plasmid DNA
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Generation of Recombinant Influenza Virus from Plasmid DNA

Generation of Recombinant Influenza Virus from Plasmid DNA

29,857 Views

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11:31 min

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August 03, 2010

DOI:

10.3791/2057-v

DOI:
10.3791/2057-v

•

11:31 min
August 03, 2010

•

29 Views
,
1Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, 2Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute,Mount Sinai School of Medicine

Summary

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Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.

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Recombinant Influenza VirusPlasmid DNAReverse GeneticsInfluenza ResearchInfectious VirusesViral ProteinsMutationsViral ReplicationPathogenesisViral VectorsForeign Proteins

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Cite this Article

Martínez-Sobrido, L., García-Sastre, A. Generation of Recombinant Influenza Virus from Plasmid DNA. J. Vis. Exp. (42), e2057, doi:10.3791/2057 (2010).

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