Single Cell Sampling Using Micromanipulation: A Technique to Isolate Single Cells from Cancer Cell Suspension

For micromanipulation, transfer the entire 100 microliters of cell suspension onto a glass slide. Then, place the glass slide on a microscope equipped with a micromanipulator and allow the cells to settle for 5 minutes. Then, in 0.2-milliliter PCR tubes, pipet 2 microliters of cell lysis master mix and transfer on ice. Use the micromanipulator to collect a single cell in 1 microliter of 1X phosphate-buffered saline.

Then, transfer the cell directly into 2 microliters of cell lysis master mix. Use a desktop microfuge to quickly spin down the samples at 2000 x g for 3 seconds to collect the liquid at the bottom of the tube.