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Developing Blood-Induced Microglia-Like Cells for Studying Human Brain Disorders
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Isolation of Mononuclear Cells from Whole Blood to Obtain Induced Microglia-Like Cells for Brain Research
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Isolation of Mononuclear Cells from Whole Blood to Obtain Induced Microglia-Like Cells for Brain Research

Isolation of Mononuclear Cells from Whole Blood to Obtain Induced Microglia-Like Cells for Brain Research

160 Views

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02:08 min

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September 06, 2024

DOI:

10.3791/201869-v

DOI:
10.3791/201869-v

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02:08 min
September 06, 2024

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25 Views
, , ,
1Department of Neuropsychiatry, Graduate School of Medical Sciences,Kyushu University, 2Department of Functional Anatomy and Neuroscience,Asahikawa Medical University

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Mononuclear CellsWhole BloodInduced Microglia-like CellsDensity Gradient MediumCentrifugationBuffy CoatPlasma RemovalPBSCell StrainerCell SuspensionIsolation MediumCell EnumerationIsolation Buffer

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Isolation of Mononuclear Cells from Whole Blood to Obtain Induced Microglia-Like Cells for Brain Research. J. Vis. Exp. (Pending Publication), e201869, doi:10.3791/201869 (2024).

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