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May 24, 2010
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在这个视频文章中，我们目前的方法隔离单个或多个<em>果蝇</em> DA神经元使用红外线（IR）的捕获激光捕获显微切割（LCM）的类三龄幼虫。从孤立的神经元获得的RNA可以很容易地用于下游应用，包括QRT - PCR或芯片分析。
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Laser Capture MicrodissectionDrosophila Peripheral NeuronsDendritic ArborizationDa NeuronsMolecular MechanismsClass-specific Dendrite MorphogenesisRNA Isolation TechniquesMagnetic Bead-based Cell PurificationFluorescent Activated Cell Sorting (FACS)RNA Binding Protein-based StrategiesLaser Capture Microdissection (LCM)Spatial PrecisionQRT-PCRMicroarray Expression ProfilingThird Instar Larval Stage
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Iyer, E. P. R., Cox, D. N. Laser Capture Microdissection of Drosophila Peripheral Neurons. J. Vis. Exp. (39), e2016, doi:10.3791/2016 (2010).
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