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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei
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Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living Trypanosoma brucei
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Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living Trypanosoma brucei

Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living Trypanosoma brucei

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02:15 min

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January 19, 2024

DOI:

10.3791/201369-v

DOI:
10.3791/201369-v

•

02:15 min
January 19, 2024

•

1 Views
, , , , , ,
1Department Chemistry and Biochemistry,Brigham Young University, 2Eukaryotic Pathogens Innovation Center,Clemson University, 3Department of Genetics and Biochemistry,Clemson University, 4Department of Physics and Astronomy,Clemson University

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Flow CytometryGlycosomePHTrypanosoma BruceiPHluorinPropidium IodideThiazole RedForward ScatterSide ScatterRelative Fluorescence Intensity

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Call, D., Pizarro, S. S., Tovey, E., Knight, E., Baumgardner, C., Christensen, K. A., Morris, J. C. Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living Trypanosoma brucei. J. Vis. Exp. (Pending Publication), e201369, doi:10.3791/201369 (2024).

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