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使用油脂介孔结构的测定结晶膜蛋白
JoVE 신문
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JoVE 신문 생물학
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
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使用油脂介孔结构的测定结晶膜蛋白

DOI:
10.3791/1712-v

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22:00 min

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November 21, 2010

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1Membrane Structural and Functional Biology Group, Schools of Biochemistry and Immunology and Medicine,Trinity College Dublin

Chapters

  • 00:00Introduction
  • 02:48Materials
  • 03:48Preparing the Crystallization Plate
  • 05:12Preparing the Lipid and Protein
  • 13:53Loading the Dispensing Syringe
  • 17:57Dispensing
  • 21:10Crystals

Summary

자동 번역

자동 번역

此处所描述的卡弗里膜的结构与功能的生物集团成立手动油脂介孔膜蛋白的结晶试验实施的过程。

Tags

Crystallizing Membrane ProteinsLipidic MesophasesLipidic Cubic PhaseIn Meso MethodX-ray Crystallographic StructuresProkaryotic ProteinsEukaryotic ProteinsMonomeric ProteinsHomo-multimeric ProteinsHetero-multimeric ProteinsChromophore-containing ProteinsChromophore-free ProteinsAlpha-helical ProteinsBeta-barrel ProteinsBeta2-adrenergic ReceptorAdenosine A2a ReceptorReconstituting Membrane ProteinMonoolein-based MesophaseManual Crystallization SetupCrystal Harvesting

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