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Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
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October 26, 2009
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We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.
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Khetan, S., Burdick, J. Cellular Encapsulation in 3D Hydrogels for Tissue Engineering . J. Vis. Exp. (32), e1590, doi:10.3791/1590 (2009).
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