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Chapters
- 00:00Title
- 00:09Introduction
- 01:05Preparing Mouse Embryonic Fibroblast (MEF) Conditioned Medium
- 02:39Transducing Human-Foreskin-Fibroblast (BJ) Cells with Stem-Cell-Specific Transcription Factors
- 06:33Selecting and Passaging Induced Pluripotent Stem Cell Colonies
- 07:33Staining Resulting Colonies for Markers of Pluripotency
- 09:43Representative Results: Induced Pluripotent Stem Cell Colonies Morphologically Resemble Stem-Cells and Express Markers of Pluripotency
- 11:07Conclusion
Vi visar protokollet för generering av inducerade pluripotenta stamceller från mänskliga somatiska celler med lentivirus-medierade leverans av mänskliga faktorer Oct4, Sox2, NANOG och Lin28. Pluripotens bekräftades av morfologi och förekomst av embryonala stamceller (ES) cell-specifika markörer.
Tags
Induced Pluripotent Stem CellsReprogrammingHuman FibroblastsStemgent Human TF Lentivirus SetRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Pluripotent StateMouse FibroblastsHuman Somatic CellsIPS CellsTranscription FactorsRetroviral VectorsJames ThomsonNanogLin28ES CellsMorphologyProliferationDifferentiationPatient-specific IPS CellsPersonalized Regenerative Medicine Therapies
