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December 08, 2009
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We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific markers.
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Induced Pluripotent Stem CellsReprogrammingHuman FibroblastsStemgent Human TF Lentivirus SetRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Pluripotent StateMouse FibroblastsHuman Somatic CellsIPS CellsTranscription FactorsRetroviral VectorsJames ThomsonNanogLin28ES CellsMorphologyProliferationDifferentiationPatient-specific IPS CellsPersonalized Regenerative Medicine Therapies
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Wu, D., Hamilton, B., Martin, C., Gao, Y., Ye, M., Yao, S. Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set. J. Vis. Exp. (34), e1553, doi:10.3791/1553 (2009).
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