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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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July 10, 2010
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PurificationSpecific Cell PopulationFluorescence Activated Cell SortingFACSTechniquePhenotypeBulk MethodsPanningComplement DepletionMagnetic Bead SeparationAdvantagesHigh PurityIdentifying MarkerDifferential Marker DensityInternal StainingIntracellular Protein ExpressionGenetically Modified Fluorescent Protein MarkerSizeGranularityFluorescenceFluorescently-tagged Monoclonal AntibodiesNegative SelectionFlow CytometerSorting CapacitySoftware
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Basu, S., Campbell, H. M., Dittel, B. N., Ray, A. Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS). J. Vis. Exp. (41), e1546, doi:10.3791/1546 (2010).
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