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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

DOI:
10.3791/1546-v

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15:29 min

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July 10, 2010

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, , ,
1Blood Research Institute,BloodCenter of Wisconsin

Chapters

  • 00:05Title
  • 00:08Introduction
  • 00:38Materials and Preparation for FACS
  • 02:17Cell Staining & Prep of Control Samples
  • 07:11Flow Cytometer Setup
  • 08:56Flow Cytometer Compensation
  • 11:58Experimental Sample Gating & Sorting
  • 14:46Conclusion

Summary

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For many scientific studies requiring a biological and chemical analysis of cell populations the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations.

Tags

PurificationSpecific Cell PopulationFluorescence Activated Cell SortingFACSTechniquePhenotypeBulk MethodsPanningComplement DepletionMagnetic Bead SeparationAdvantagesHigh PurityIdentifying MarkerDifferential Marker DensityInternal StainingIntracellular Protein ExpressionGenetically Modified Fluorescent Protein MarkerSizeGranularityFluorescenceFluorescently-tagged Monoclonal AntibodiesNegative SelectionFlow CytometerSorting CapacitySoftware

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