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表面固定生物分子的单分子荧光检测的自动化系统
Chapters
- 00:00Title
- 01:44Introduction
- 02:12Assembling the Microfluidic Cell
- 05:35Activating the Surface of the Microfluidic Cell for Molecule Immobilization
- 07:31Preparing the Imaging Buffer and Scanning the Sample
- 10:03Time-Resolved Single Molecule FRET Traces of DNA Molecules
- 10:31Conclusion
Tags
Automated SystemSingle Molecule Fluorescence MeasurementsSurface-immobilized BiomoleculesFluorescence Resonance Energy Transfer MicroscopyFRET MicroscopyNucleic AcidsProteinsSm-FRET MeasurementsTime-tracesBiomolecular DynamicsStatistical AnalysesAutomated Scanning Confocal MicroscopeMicrofluidic CellBuffer ExchangeOxygen ContentConstant TemperatureMicrofluidic Device AssemblyDNA ImmobilizationBuffer PreparationPhotostabilityFluorophoresSetup Preparation
