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November 02, 2009
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Automatically generated
In this article we describe how we obtain FRET traces from individual DNA molecules immobilized to a surface using an automated scanning confocal microscope.
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Automated SystemSingle Molecule Fluorescence MeasurementsSurface-immobilized BiomoleculesFluorescence Resonance Energy Transfer MicroscopyFRET MicroscopyNucleic AcidsProteinsSm-FRET MeasurementsTime-tracesBiomolecular DynamicsStatistical AnalysesAutomated Scanning Confocal MicroscopeMicrofluidic CellBuffer ExchangeOxygen ContentConstant TemperatureMicrofluidic Device AssemblyDNA ImmobilizationBuffer PreparationPhotostabilityFluorophoresSetup Preparation
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Di Fiori, N., Meller, A. Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules. J. Vis. Exp. (33), e1542, doi:10.3791/1542 (2009).
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