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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
JoVE 신문
생물학
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JoVE 신문 생물학
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

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14:36 min

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August 26, 2009

DOI:

10.3791/1432-v

DOI:
10.3791/1432-v

•

14:36 min
August 26, 2009

•

1 Views
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1Mechanical Engineering,Johns Hopkins University, 2Biomedical Engineering,Duke University, 3Biomedical Engineering,Johns Hopkins University

Summary

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We present a novel and powerful integration of nanophotonics (QD-FRET) and microfluidics to investigate the formation of polyelectrolyte polyplexes, which is expected to provide better control and synthesis of uniform and customizable polyplexes for future nucleic acid-based therapeutics.

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QD-FRETMicrofluidicsDNA Nanocomplex Self-assemblyGenomicsTherapeuticsIntracellular Delivery SystemGene CarrierElectrostatic Self-assemblyCellular UptakeDegradation ProtectionRational DesignPremature DissociationStable BindingSelf-assembly KineticsPhysical PropertiesTransfection EfficienciesBioactivitiesNanophotonics

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Ho, Y., Chen, H. H., Leong, K. W., Wang, T. Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time. J. Vis. Exp. (30), e1432, doi:10.3791/1432 (2009).

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