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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

37,225 Views

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05:18 min

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December 11, 2008

DOI:

10.3791/1029-v

DOI:
10.3791/1029-v

•

05:18 min
December 11, 2008

•

30 Views
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1Miltenyi Biotec,GmbH

Summary

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This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

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Jungblut, M., Oeltze, K., Zehnter, I., Hasselmann, D., Bosio, A. Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator. J. Vis. Exp. (22), e1029, doi:10.3791/1029 (2008).

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