Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus
Summary
In this video, we demonstrate two microapplicator techniques used to infect of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.
Abstract
Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.
Protocol
Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the microapplicator techniques used in this assay.
Disclosures
The authors have nothing to disclose.
Cite This Article
Li, H., Sparks, W., Bonning, B. Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (18), e889, doi:10.3791/889 (2008).