A novel approach is presented to induce chronic dry eye disease in rabbits by surgically removing all orbital lacrimal glands. This method, distinct from those previously reported, produces a stable, reproducible model of aqueous deficient dry eye well suited to study tear physiology and pathophysiology and ocular therapeutics.
Dry eye disease (DED) is a complex disease with multiple etiologies and variable symptoms, having ocular surface inflammation as its key pathophysiologic step. Despite advances in our understanding of DED, significant knowledge gaps remain. Advances are limited in part due to the lack of informative animal models. The authors recently reported on a method of DED induced by injecting all orbital lacrimal gland (LG) tissues with the lectin concanavalin A. Here, we report a novel model of aqueous-deficient DED based on the surgical resection of all orbital LG (dacryoadenectomy) tissues. Both methods use rabbits because of their similarity to human eyes in terms of the size and structure of the ocular surface. One week after removal of the nictitating membrane, the orbital superior LG was surgically removed under anesthesia, followed by removal of the palpebral superior LG, and finally removal of the inferior LG. Dacryoadenectomy induced severe DED, evidenced by a marked reduction in the tear break up time test and the Schirmer's tear test, and significantly increased tear osmolarity and rose bengal staining. Dacryoadenectomy-induced DED lasted at least eight weeks. There were no complications and animals tolerated the procedure well. The technique can be mastered relatively easily by those with adequate surgical experience and appreciation of the relevant rabbit anatomy. Since this model recapitulates the features of human aqueous-deficient DED, it is suitable for studies of ocular surface homeostasis, DED, and candidate therapeutics.
Tears are required for the protection of the ocular surface and for the maintenance of the optical properties of the cornea. They consist of three layers: an inner mucin coating, a middle aqueous component, and a lipid overlay1. The mucin layer is produced predominantly in goblet cells of the conjunctiva, the aqueous component predominantly in the lacrimal glands (LGs), and the lipid layer predominantly in the Meibomian glands1,2. The orbital LGs are the main source for the aqueous component of tears and for many of the proteins that protect the surface from bacterial attack3. Ocular surface diseases ensue when the aqueous tear production is decreased below a critical level, depriving the epithelial surfaces of the eye of the aqueous component and crucial tear constituents including growth factors, lysozyme, and lactoferrin. In cases of decreased tear production by the LGs, the conjunctival and corneal tissues undergo adaptations to compensate for the altered environment.
Understanding the contribution of the tear component derived from the orbital LGs and the ocular surface's compensatory mechanisms when this is lacking impacts our appreciation of the physiology and pathophysiology of the anterior segment of the eye and, more broadly, of health and disease in the entire globe. The experimental approach to these issues requires an informative animal model. Consequently, several groups have attempted to develop animal models in which the orbital LGs are removed, thereby facilitating the assessment of the role of tears in ocular health. One such model was recently reported for the mouse4. The rabbit offers, however, many distinct advantages over rodent models including similar anatomic and histologic structures of the LG, and perhaps more importantly, similar size and surface area of the cornea and conjunctival tissues when compared to their human counterparts3.
Creation of aqueous deficient dry eye disease (DED) by surgical resection of LG tissue in rabbits is not new. Numerous reports describe resection of LG tissues with varying success reflected in variable changes in tear production measured by the Schirmer's tear test5,6,7,8. A thorough understanding of the relevant anatomy of the rabbit and clarity about the anatomical terminology are very helpful in reproducing this method. A thorough overview of both is provided below.
Anatomy of the lacrimal glands
The rabbit has two orbital LGs: the larger inferior LG (ILG) and the smaller superior LG (SLG; Figure 1). The ILG extends along the inferior and posterior aspect of the orbital rim. With the exception of variable size, the anterior portion of the ILG has a fairly uniform bulbous appearance that can be seen as a protuberance in the skin under the globe (Figure 2). Because of its characteristic appearance in relation to the rest of the gland, it is referred to as the "head" of the ILG. A portion of the head wraps around and lies on the external surface of the zygomatic bone. This serves as a useful landmark on ultrasound biomicroscopy to guide injections into the ILG. The remainder of the head resides more medially9 in the orbit.
Due to the characteristic appearance of the remaining portion of the ILG, which is long and thin, this segment is referred to as the "tail." The tail runs along the inferior orbital rim, from the head of the ILG to the orbital rim where it terminates with variable anatomy at the inferior and posterior orbital rim (Figure 3A). The tail lies deep (medial) to the zygomatic bone separated from the orbital contents by a fascial band for most of its course until it reaches the posterior rim of the orbit where it once again extends out over the external surface of the zygomatic bone. The ILG receives its blood supply from branches of the carotid artery.
The SLG has two components analogous to the human. One is the palpebral superior LG (PSLG), which resides in the upper posterior eyelid medial to the tarsal plate. It appears bulbous in nature and has numerous punctate openings that drain aqueous tear fluid that is more easily seen when covered with 2% fluorescein (Figure 3B).
The second is the orbital superior LG (OSLG), residing in a medial position in the superior orbit (Figure 3C). Due to its position near the midline of the skull, it has been impossible to successfully identify it using external surgical approaches from the temporal or inferior orbit. In fresh necropsy samples or surgical cases, this gland can be prolapsed through the posterior incisure located in the dorsal surface of the skull when gentle medial pressure is applied to the globe. Prolapse of this glandular tissue can be documented with ultrasound biomicroscopy.
The PSLG and OSLG are contiguous structures. The OSLG is a tubuloalveolar structure whose ductal architecture coalesces into the main excretory duct. This duct passes under the supra-orbital ridge and runs in the upper lid tissues terminating in the PSLG. Along the excretory duct, glandular tissue consistent with the original descriptions of Davis has been identified10 (Figure 3D).
A note on terminology
Excellent and comprehensive anatomic descriptions use varying terminology as well. The classic orbital anatomy by Davis defines only an upper and lower LG10. However, his description of the upper LG clearly details the portions more specifically defined here as the PSLG and OSLG, while his description of the lower LG details the portions defined here as the head and tail of the ILG. A more recent and thorough anatomical atlas11 defines these tissues as the zygomatic gland and the accessory LG. The term "lacrimal gland" is used here to comprise the aforementioned PSLG and OSLG. This terminology is better suited for reproducing this method without undue confusion.
All vertebrate animal studies were completed in accordance and compliance with all relevant regulatory and institutional guidelines. All studies were approved by the Institutional Review Board of Stony Brook University and performed in accordance with the Association for Research in Vision and Ophthalmology (ARVO) statement for the use of animals in ophthalmic and vision research.
1. Animals and housing
2. Removal of the nictitating membrane
NOTE: For simplicity, the technique for the right eye is described below. Complete this procedure on the left eye in an identical manner.
3. Measurement of dry eye parameters and collection of tear samples
4. Surgical preparation and anesthesia
5. Complete surgical dacryoadenectomy
NOTE: The complete surgical dacryoadenectomy, as described herein, was done using 0.3 tissue forceps, tenotomy scissors, non-toothed tissue forceps, and scissors. These instruments can be interchanged with similar instruments that perform the same function based on surgeon preference.
6. Postprocedural care
The complete dacryoadenectomy method described here was performed on 8 animals. It requires a moderate degree of surgical skill. Surgical time averaged about 2.2 h for bilateral surgery, excluding the removal of the nictitating membrane, which was done separately and required <10 minutes. There were no fatalities or intraoperative complications and no rabbit required any hemostatic aid other than modest cautery.
Our surgical approach successfully induced dry eye in all eyes. This was confirmed by a panel of clinical and laboratory markers of DED (Table 1). During the 8 weeks of observation, the mean TBUT was suppressed by more than 75% of preoperative levels (p < 0.0001 for all time points). Similarly, the Schirmer's tear test decreased by approximately 50%, remaining so for the 8 weeks of observation; it showed no trend for recovery during the follow up period. Postoperatively, tear osmolarity showed a 10% increase consistent with DED, sustained for at least 8 weeks postoperatively. Rose bengal staining of the cornea also increased and did not show signs of recovery during the 8 weeks of follow-up (Figure 8). All eyes undergoing complete dacryoadenectomy showed marked reduction in goblet cell numbers and epithelial changes consistent with dry eye (conjunctival impression cytology).
Figure 1: Rabbit lacrimal gland anatomy (right eye). The orbital superior lacrimal gland (OSLG) is comprised of a larger orbital part and smaller palpebral component. The larger inferior lacrimal gland (ILG) is comprised of the anterior/head and posterior/tail portions. Coordinate axes show the terminology used for all descriptions of orientation used within the text. Please click here to view a larger version of this figure.
Figure 2: Location of the head of the ILG. The lateral view of the right face after removal of fur. A bulge in the skin contour (indicated by thick arrows) inferior to the anterior orbit indicates the location of the head of the ILG that lies on the external surface of the zygomatic bone at this location. Please click here to view a larger version of this figure.
Figure 3: The orbital lacrimal glands. (A) The right inferior lacrimal gland (ILG) after staining with Evans blue dye showing the close proximity of the tail of the ILG (red arrow) just medial to the zygomatic bone (black arrow) and inferior to the globe. (B) Tear production from the palpebral superior lacrimal gland (PSLG). Time-lapse photos taken after topical application of 2% fluorescein. Aqueous fluid emanating from the PSLG dilutes the initially dark blue or black fluorescein dye turning it bright yellow green (similar to Seidel testing). (C) Position of orbital SLG (OSLG) in the rabbit skull, lying close to the midline of the skull (dotted line) within the posterior incisure (arrow). Evans blue dye was injected into the OSLG and palpebral superior lacrimal gland. (D) Histology section through the main excretory duct of the OSLG surrounded by a small amount of glandular tissue (arrow) is seen in this histopathologic cross-section stained with hematoxylin and eosin dyes taken through the posterior (temporal) aspect of the upper right eyelid. Please click here to view a larger version of this figure.
Figure 4: Surgical site preparation. (A) Upper left panel: Removal of long fur with shears. All residual fine fur is subsequently removed with a mild depilatory cream. Upper right panel: Final appearance following complete fur removal that allows for surgical marking and high-quality ultrasound of the ILG to be performed. (B) Appropriate surgical markings of the right periorbital region are shown; in this example, the incisions to remove the OSLG and ILG have been connected to create one long curvilinear incision. The location of the posterior incisure is indicated by a small hash mark on the curvilinear incision site marking (arrow). Please click here to view a larger version of this figure.
Figure 5: Removal of the OSLG. (A) Surgical sites are infiltrated with anesthetic using a 50:50 combination of 2% lidocaine with 1:100,000 epinephrine and 0.5% bupivacaine, which is injected into the upper lid and along the incision lines to minimize discomfort during the procedure. (B) A Colorado microdissection needle is used to incise the skin and superficial muscle layers along the pre-marked surgical incision sites. Gentle traction across the wound is applied to help create the dissection plane. The small pinpoint burns (arrow) were made with the Colorado needle at equidistant points along the incision line to help optimally realign the tissues during wound closure. (C) The OSLG is exposed after tissues overlying the posterior incisure have been mobilized (arrow). The capsule of the gland has been incised. The OSLG can be prolapsed by applying medial pressure to the globe facilitating its removal. (D) Forceps are used to engage the OSLG and gently remove it from its deeper position within the orbit through the posterior incisure. Please click here to view a larger version of this figure.
Figure 6: Removal of the palpebral superior lacrimal gland (PSLG) and excretory duct. (A) Following eversion of the upper eyelid, the bulbous portion of the PSLG is engaged with forceps and dissected off the tarsus using scissors. Traction applied to the PSLG with forceps is critical to maintaining the surgical plane. (B) The dissection of the PSLG and the main lacrimal duct is carried superiorly toward the orbital rim using sharp dissection and continuous traction on the gland and duct tissues to maintain the appropriate surgical plane. The dissection should proceed to the point where the OSLG was removed. Please click here to view a larger version of this figure.
Figure 7: Removal of the ILG. (A) The skin and superficial muscle are incised until the fascial plane overlying the zygomatic bone or superficial part of the masseter muscle is reached. The head of the ILG usually is clearly evident as a small bulge located under the anterior limbus. (B) The fibrous capsule of the ILG is incised with scissors exposing the ILG. Once the capsule is incised, the deeper portions of the gland can be easily removed. (C) The most external portion of the ILG head that lies on the zygomatic bone has been exposed and reflected anteriorly showing the underlying zygomatic bone. (D) Incision of the orbital septum along the inferior rim exposes the tail of the ILG. (E) A branch of the external carotid artery feeds the tail of the ILG (arrow). (F) Appearance following the closure of skin incisions after complete dacryoadenectomy. Please click here to view a larger version of this figure.
Figure 8: Rose Bengal staining of the corneal surface. External photograph showing prominent staining, most notable on the nasal quadrant. All eyes undergoing complete dacryoadenectomy developed similar changes that were evident by 1 week after surgery and persisted for at least 6 weeks. Of note, the light reflex from the ring flash shows distortion from a dry ocular surface demonstrating how dry eye can adversely impact vision. Please click here to view a larger version of this figure.
Dacryoadenectomy | ||
mean ± SEM; n = 16 eyes | ||
Baseline | Week 2 | |
Tear break-up time, s | 60.0 ± 0.0 | 4.5 ± 1.2 |
p < 0.0001 | ||
Tear osmolarity, mOsm | 291.2 ± 3.7 | 315.3 ± 5.5 |
p = 0.001 | ||
Schirmer tear test, mm | 18.3 ± 1.3 | 10.5 ± 1.6 |
p = 0.0006 | ||
Rose bengal, modified NEI score | 0.0 ± 0.0 | 4.28 ± 0.6 |
p < 0.0001 | ||
Operated vs. baseline: Dacryoadenectomy: TBUT, p < 0.0001; tear osmolarity, p < 0.001; Schirmer tear test, p < 0.0006); and rose bengal. |
Table 1: Dry eye parameters on postoperative week 2.
DED is classified into two major groups based on the effect on tear film stability: aqueous deficient (decreased production of the aqueous component of the tear film; ~20% of DED) and evaporative (increased evaporation of the tear film; ~50% of DED). About 30% of DED patients show evidence of both (mixed DED). Inflammation is the core mechanism of DED to which its diverse etiologies converge13,14. Our method models aqueous-deficient DED.
As previously mentioned, important first steps in reproducing our method are an appreciation of the fine points of the anatomy of the orbital lacrimal glands (LGs) of the rabbit and avoiding confusion by varied and sometimes conflicting anatomical terminology. The anatomical atlas by Popesko et al.11 is extremely thorough. For those less comfortable with the anatomy of the rabbit, dissection of necropsy specimens provides easy familiarity with these structures and aids their surgical removal in living specimens.
Critical advice on animal housing and acclimation has been given in our companion publication12. The same article also presents useful comments for assaying the parameters of DED used in both methods.
In contrast to the previous method12, this one requires a higher level of surgical skill because of the extent and more invasive nature of the techniques needed to remove the LGs. The greatest risk during these resections is catastrophic bleeding caused by injuring major vessels that are in close proximity to the LGs such as branches of the carotid artery. This is avoided by adequately visualizing each LG and its margins within the surgical field. Finally, overzealous removal of the nictitating membrane could lead to the prolapse of the Harderian gland, which can disrupt tear film assessment.
Care should be taken to minimize the amount of conjunctival disruption with the removal of the PSLG, a novel aspect of our method that improves reproducibility and enhances the severity of DED. It is surprisingly easy to establish the dissection plane and carry it back to the superior orbital ridge as long as traction is applied to the tissues. It is reassuring to be able to see the cautery marks from the truncation of the OSLG; they confirm the complete removal of the main excretory duct of the gland.
Removal of the ILG in its entirety presents challenges as well. Isolate the head of the gland first, as this is the easiest part to visualize. The entire head of the gland tissue separates easily from the surrounding tissues; however, some care must be used to prevent damage to the large venous sinus, which lies medial to the head of the ILG. The tail of the ILG can then be followed back as it passes under the zygomatic bone. The majority of the tail is easy to isolate. However, the most posterior aspect of the tail can prove more challenging because of variable anatomy and its proximity to a medium-sized branch of the carotid. Careful dissection should allow all margins of the ILG to be seen clearly, facilitating its complete removal. The investigator should be prepared to carry the dissection more superiorly in cases where the tail of the gland ends under the lateral canthus, as explained in the earlier discussion of the anatomy of the lacrimal glands. Of note, the authors have never been able to identify any portion of the OSLG when dissecting the ILG through a curvilinear incision along the temporal and inferior globe. Although this may be technically possible, that surgical approach carries too high a risk for serious bleeding. Approaching the OSLG through the posterior incisure proves far safer.
The excretory duct of the ILG can be seen penetrating through the inferior fascial plane as it passes into the lower conjunctival fornix. Occasionally, small lobules of glandular-appearing tissue are seen here as well and can be carefully removed.
It is very helpful to maintain the order of LG resection as presented here. If the ILG is removed first, the isolation of the OSLG becomes technically far more difficult. The main reason is that, after removal of the ILG, the OSLG cannot be easily prolapsed and thereby identified.
A significant advantage of our model is that it can be "modular." In other words, the degree of DED induced by dacryoadenectomy can be calibrated to serve experimental needs. For example, resection of all LGs would cause maximal DED, but resection of only the SLG would cause the mildest form of DED and resection of only the ILG would generate disease of intermediate severity.
Our approach, which recapitulates the distinct pathophysiological event of reduced tear production offers additional advantages compared to already reported methods. Briefly, no other surgical model eliminated tear production by all orbital LGs5,6,7,15,16; including parasympathetic denervation of the LGs17, and pharmacological suppression of tear production18,19, with the latter two having their off-target effects as significant confounders. Finally, this model minimizes the main investigator-dependent bias, namely the incomplete resection of the LGs, since the surgical technique affords their complete visualization; this is aided by the fact that no hemostasis, other than cautery, is required.
The investigator should be cognizant that complete resection of all orbital LGs does not generate complete absence of tears, and, for example, Schirmer's tear test values approaching zero should not be expected. This is due to the fact that there are always other sources of tear fluid such as the accessory LGs of Wolfring and Krause and plasma leakage from conjunctival vessels20,21,22. From an experimental standpoint, this should be viewed as a positive aspect of the method as it maintains the ocular surface; complete xerophthalmia would totally destroy the cornea negating the usefulness of the model. Additionally, in its current embodiment, this model offers an excellent opportunity to study such compensatory mechanisms and fluid transport across these smaller compartments.
In conclusion, presented here are the specifics of a novel and versatile method of inducing aqueous-deficient DED that lends itself to the study of tear physiology, the pathogenesis of DED and the study of therapeutic agents being developed for this indication.
The authors have nothing to disclose.
We acknowledge the financial support from a Targeted Research Opportunities grant from the Stony Brook University School of Medicine and a research grant from Medicon Pharmaceuticals, Inc., Setauket, NY. We thank Michele McTernan for editorial support.
acepromazine, Aceproinj | Henry Schein Animal Health, Dublin, OH | NDC11695-0079-8 | 0.1ml/kg subcutaneously injection for rabbit sedation |
anesthesia vaporizer | VetEquip, Pleasanton, CA | Item # 911103 | Protocol 4.8 |
animal restraining bag | Henry Schein Animal Health, Dublin, OH | Jorvet J0170 | Use appropriately sized bag. |
bupivacaine, 0.5% | Hospira Inc, Lake Forest IL | NDC: 0409-1162-02 | Mixed 50:50 with 2% lidocaine with 1:100,000 epinephrine for infiltration of incision sites, protocol 5.1 |
buprenorphine | Henry Schein Animal Health, Dublin, OH | 0.01 mg/kg, for postprocedural care, 6.1.4 | |
cautery unit, high-temperature, battery-powered | Medline Industries Inc, Northfield, IL | REF ESCT001 | Keep on hand in case of bleeding, protocol 2.7 |
clipper, Wahl Mini Arco | Henry Schein Animal Health, Dublin, OH | No. 022573 | Cordless shears for fur removal, protocol 4.2 |
Colorado needle | Stryker Craniomaxillofacial, Kalamazoo, MI | N103A | Use with electrosurgical unit to make incisions, protocol 5.1 & 5.3 |
electrosurgical unit with monopolar cautery plate | Valleylab, Boulder, CO | Force FXc | Use with electrosurgical unit to make incisions, protocol 5.1 & 5.3 |
fluorescein, Ak-Fluor 10% | AKRON, Lake Forest, IL | NDC17478-253 | Dilute to 0.2% with PBS to measure TBUT, measurement of dry eye parameters, protocol 3.1 |
foceps, curved dressing | Bausch and Lomb (Storz), Bridgewater, NJ | Storz E1406 | delicate serrated dressing forceps |
forceps, 0.3 | Bausch and Lomb (Storz), Bridgewater, NJ | ET6319 | For removal of nictating membrane, protocol 2.5 |
forceps, Bishop Harmon | Bausch and Lomb (Storz), Bridgewater, NJ | E1500-C | Use toothed forceps for dacryoadenectomy, protocol 5.1 & 5.2 |
hair remover lotion, Nair | Widely available | Softening Baby oil | Dipilitory cream for sensitive skin, protocol 4.2 |
isoflurane | Henry Schein Animal Health, Dublin, OH | 29405 | Possible alternative sedation, protocol 4.7 |
IV catheter, Terumo Surflo ETFE 24-gauge | Terumo, Tokyo, Japan; available from Fisher Sci., VWR, McKesson, etc. | SR-OX2419CA | 25-gauge for smaller rabbits; protocol 4.6 |
ketamine | Henry Schein Animal Health, Dublin, OH | NDC 11695-0701-1; NADA 200-055 | 15 mg/kg, protocol 4.7 |
ketoprofen | Hospira, Inc., Lake Forest, IL | 3 mg/kg, for postprocedural care, 6.1.4 | |
laryngeal mask airway | Docsinnovent Ltd, London, UK | Vgel R3 | Protocol 4.8 |
lid speculum, wire | Bausch and Lomb (Storz), Bridgewater, NJ | Barraquer SUH01 | For removal of nictating membrane, protocol 2.4 |
lidocaine 2% with epinephrine 1:100,000; 50:50 mixture | Hospira Inc, Lake Forest IL | NDC 0409-3182-02 | Pre-treat before removal of nictating membranes, protocol 2.4 |
lidocaine, preservative-free | Sigma-Aldrich, St. Louis, MO | L5647 | 1% in PBS for anesthesia agent, for application to eye, protocol 2.4 |
micropipette | Eppendorf | Research Plus 100 uL | For application of preservative-free lidocaine to eye, protocol 2.4 |
micropipette tips | World Wide Medical Products | 41071052 | For application of preservative-free lidocaine to eye, protocol 2.4 |
monitoring device, multi-parameter | SurgiVet, Waukesha, WI | V9201 | For monitoring of vitals, protocol 4.9 |
needle, 26-gauge | BD, Franklin Lakes, NJ | REF 305115 | For injection of lidocaine/epinephrine, protocol 2.3 & 2.5 |
needle, 30-gauge | BD, Franklin Lakes, NJ | REF 305106 | For infiltration of incision sites; syringe and needle size are not critical, protocol 5.1 |
osmolarity tips | TearLab Corp., San Diego, CA | #100003 REV R | Measure tear osmolarity measurement of dry eye parameters, protocol 3.1 |
osmometer, TearLab | TearLab Corp., San Diego, CA | Model#200000W REV A | Measure tear osmolarity, measurement of dry eye parameters, protocol 3.1 |
povidone-iodine solution | Medline Industries Inc, Northfield, IL | PVP Prep Solution, NDC: 53329-939-04, REF MDS 093944 | To maintain sterile field, protocol 4.11 |
rabbit, New Zealand White | Charles River Labs, Waltham, MA (NZW) | 2-3 kg | Research animals |
Rose bengal stain | Amcon Laboratories Inc., St. Louis, MO | NDC51801-004-40 | 1% in PBS, for staining the ocular surface, measurement of dry eye parameters, protocol 3.1 |
saline, normal | B. Braun Medical, Irvine, CA | REF R5200-01 | For postprocedural care, protocol 6.1.3 |
Schirmer Tear Test strips | Eaglevision, Katena products. Denville, NJ | AX13613 | Measure tear production, measurement of dry eye parameters, protocol 3.1 |
scissors, Vannas | McKesson Medical-Surgical, San Francisco, CA | Miltex 2-130 | Capsulotomy scissors for dacryoadenectomy, protocol 5.1 & 5.2 |
scissors, Westcott tenotomy | McKesson Medical-Surgical, San Francisco, CA | Miltex 18-1480 | For removal of nictating membrane, protocol 2.7 |
sedation gas mask | DRE Veterinary, Louisville, KY | #1381 | Possible alternative sedation, protocol 4.7 |
surgical marking pen | Medical Action Industries, Arden, ND | REF 115 | Protocol 4.2 |
sutures, 5-0 Mersilene | Ethicon US, LLC | Ethylene terephthalate sutures, used for deep connective tissue closure, protocol 5.3.11 | |
sutures, Vicryl 6-0 | Ethicon US, LLC | Polyglactin 910 sutures, used for superficial muscle and skin closure, protocol 5.3.11 | |
syringe, 1 cc | BD, Franklin Lakes, NJ | ref 309659 | For injection of lidocaine/epinephrine, protocol 2.3 & 2.5 |
syringe, 5 cc | BD, Franklin Lakes, NJ | REF 309603 | For infiltration of incision sites; syringe and needle size are not critical, protocol 5.1 |
tissue forceps, 0.8mm Graefe | Roboz Surgical Store, Gaithersburg, MD | RS-5150 | Curved Weck forceps |
topical antibiotic ointment (neomycin, polymyxin, bacitracin, and hydrocortisone) | Bausch and Lomb, Tampa, FL | NDC 24208-785-55 | Applied after removal of nictating membrane, protocol 2.8, and for postprocedural care, protocol 6.1.2 |
ultrasound gel | Parker Laboratories, Inc., Fairfield, NJ | Aquasonic 100 | To ensure electrical contact with monopolar cautery plate, protocol 4.5 |
xylazine | Henry Schein Animal Health, Dublin, OH | NADA: 139-236 | 1 mg/kg, protocol 4.7 |