Development of a 3D Graphene Electrode Dielectrophoretic Device

Hongyu Xie; Radheshyam Tewari; Hiroyuki Fukushima; Jeffri Narendra; Caryn Heldt; Julia King; Adrienne R. Minerick

doi:10.3791/51696

JoVE Journal > Engineering

공학

Development of a 3D Graphene Electrode Dielectrophoretic Device

Published: June 22, 2014

doi:

10.3791/51696

Hongyu Xie¹, Radheshyam Tewari², Hiroyuki Fukushima³, Jeffri Narendra³, Caryn Heldt¹, Julia King¹, Adrienne R. Minerick¹

¹Department of Chemical Engineering,Michigan Technological University, ²Department of Mechanical Engineering,Michigan Technological University, ³XG Sciences, Inc.

Summary

A microdevice with high throughput potential is used to demonstrate three-dimensional (3D) dielectrophoresis (DEP) with novel materials. Graphene nanoplatelet paper and double sided tape were alternately stacked; a 700 μm micro-well was drilled transverse to the layers. DEP behavior of polystyrene beads was demonstrated in the micro-well.

Abstract

The design and fabrication of a novel 3D electrode microdevice using 50 µm thick graphene paper and 100 µm double sided tape is described. The protocol details the procedures to construct a versatile, reusable, multiple layer, laminated dielectrophoresis chamber. Specifically, six layers of 50 µm x 0.7 cm x 2 cm graphene paper and five layers of double sided tape were alternately stacked together, then clamped to a glass slide. Then a 700 μm diameter micro-well was drilled through the laminated structure using a computer-controlled micro drilling machine. Insulating properties of the tape layer between adjacent graphene layers were assured by resistance tests. Silver conductive epoxy connected alternate layers of graphene paper and formed stable connections between the graphene paper and external copper wire electrodes. The finished device was then clamped and sealed to a glass slide. The electric field gradient was modeled within the multi-layer device. Dielectrophoretic behaviors of 6 μm polystyrene beads were demonstrated in the 1 mm deep micro-well, with medium conductivities ranging from 0.0001 S/m to 1.3 S/m, and applied signal frequencies from 100 Hz to 10 MHz. Negative dielectrophoretic responses were observed in three dimensions over most of the conductivity-frequency space and cross-over frequency values are consistent with previously reported literature values. The device did not prevent AC electroosmosis and electrothermal flows, which occurred in the low and high frequency regions, respectively. The graphene paper utilized in this device is versatile and could subsequently function as a biosensor after dielectrophoretic characterizations are complete.

Introduction

Graphene is a novel material known for its high quality electronic properties and potential chemical and biosensor applications¹. Graphene nanoplatelets have been used for catalyst support²^,³, biosensors⁴, super-capacitors⁵, and composite-electrodes including graphene/polyaniline and silicon nanoparticle/graphene composites^6-8. This manuscript describes utilization of graphene paper as electrodes in a unique three-dimensional (3D), layered microfluidic device. Graphene paper electrodes were laminated with insulative double-sided tape and a chamber drilled within which 3D AC dielectrophoresis of polystyrene beads was performed.

Dielectrophoresis (DEP) refers to the movement of polarizable particles under non-uniform electrical fields. Positive DEP (pDEP) or negative DEP (nDEP) occurs when particles are more or less polarizable than the surrounding medium, resulting in movement toward the strongest or weakest electrical field, respectively. This nonlinear electrokinetic tool has been used for separation, sorting, trapping, and identification of particles and biological cells^9-15. The dielectrophoretic force experienced by a polarized particle is a function of the electric field gradient, particle radius and shape, particle dielectric properties including conductivity and permittivity, as well as the media conductivity and permittivity. In traditional two-dimensional (2D) DEP, particle movement is in the primary plane of the electric field gradient typically formed between microfabricated surface electrodes; movement in the vertical direction is negligible compared to in-plane directions in most devices. However, harnessing this third dimension of electrical field gradients for 3D DEP allows for higher sample throughput and increases the versatility to design new and improved dielectrophoretic separations in which the flow is traverse to the field gradients¹⁶^,¹⁷. Other specific designs include 3D insulator-based DEP¹⁸, 3D carbon-electrode DEP¹³^,¹⁹, and 3D electroplating DEP¹⁰. As evidenced by the research into 3D structures, such devices can be operated in continuous flow mode to achieve higher throughputs. Observation of the 3D particle movement in our layered 3D device is achieved as a function of frequency and medium conductivity via light microscopy at different focal heights.

Fatoyinbo et al. first reported DEP in a 3D laminated electrode/insulation structure using alternatively stacked 30 μm aluminum foil and 150 μm epoxy resin films²⁰. Hubner et al. then designed similar 3D laminated electrodes with 35 μm copper tape and 118 μm polyimide adhesive²¹. This work borrows the 3D-well design²²^,²³, and uniquely utilizes the convenience of 50 μm graphene paper as the conducting layers and 100 μm double-sided tape as the insulating layers, which achieved sealing and sufficient electrical shielding. Graphene paper versatility is a distinct advantage for 3D electrode microdevices because the graphene nanoplatelets have the ability to concurrently act as biosensors, which this group previously demonstrated²⁴.

The field gradients achieved within the graphene paper/polymer laminated 3D microdevices depend on the micro-well dimensions, the graphene paper layers, and the applied electric field. Critical dimensions include the vertical electrode spacing (conducting and insulating layer thicknesses) and micro-well diameter and height (determined by layers stacked). The electric signal can be tuned via amplitude and frequency. The current device structure is for batch operation, but can be tailored to a continuous flow device. The device fabrication technique described here is suitable for developing 3D laminated electrodes with a wide variety of graphene nanoplatelet properties simply by exchanging the graphene paper utilized. Advantages of utilizing graphene paper are versatility of physical and chemical properties, reduced expense, and the graphene nanoplatelets can concurrently act as biosensors to detect a wide range of bioanalytes²⁴. Long-term goals of high throughput 3D DEP systems are to rapidly identify cell types^25-27, or achieve label-free, electrically mediated cell sorting of diseased cells from populations of healthy cells²⁸. This paper demonstrates material optimization and device preparation and operation followed by illustration and analysis of typical results.

Protocol

1. Fabricate a Laminated Electrode/insulation 3D Structure

For a 6 graphene layer, 5 tape layer device, cut graphene paper with a scalpel or similar razor blade and straight-edged ruler into six 0.7 cm x 1.5 cm rectangles and use scissors to cut double-sided pressure-sensitive tape into five 1.3 cm x ~5 cm stripes.
NOTE: As shown in Figure 1a, this yields a 3 ground electrode, 3 AC signal electrode device. The 7 mm conducting layer width is narrow enough to fit onto a glass slide, yet wide enough for easy drilling. The 2 mm length does not easily break upon repeated use and has sufficient room to attach copper wires. The device depth is limited by end mill depths.
Lay the first layer of graphene paper on a clean glass slide. Slowly cover one end of the graphene paper with one stripe of tape, leaving a ~2 mm margin to assure insulation between the two adjacent graphene paper layers (Figure 1b).
Place the second layer of graphene paper on the top of the tape offset to the first layer of graphene paper (Figure 1a). Apply moderate pressure (press uniformly with thumb, ~100 N over 0.7 cm² area) after addition of each conducting layer to ensure good sealing between layers.
Repeat steps 1.2 and 1.3 for the remaining layers, leaving both the top and bottom layers graphene paper. Cut along the dotted line shown in Figure 1b to remove the excess tape from the device edges leaving a small ~1 mm margin to assure sealed insulation between graphene paper layers (Figure 1b).
NOTE: Double-sided tape is not utilized as the top and bottom layers to avoid collecting debris as this laminated structure is drilled, mounted on a slide, and filled with sample.
Perform a quick insulation test with a multimeter (resistance mode). Position the positive and negative probes on two different sides of the device (A and B on Figure 1c); high resistance (kilo- to mega-Ohms) indicates good insulation between layers. Remove the layered structure from the glass slide to prepare for micro-well drilling.
NOTE: A device typically fails the insulation test when adjacent graphene paper layers make contact during steps 1.2 through 1.4. Discard such devices.

2. Drill Micro-well in the Laminated Structure

Use a computer-controlled mechanical micro-milling machine and choose an end mill with a 700 µm in diameter and 2.1 mm length of cut. Immobilize the laminated structure on the micro-milling stage using appropriate clamps (Figures 2a and b). Run the milling machine spindle at 8,600 rpm, then lower the end mill slowly into and through the center of the laminated structure. Move the rotating end mill up and down through the micro-well to smooth the inside wall.
1. Choose micro-well diameters, which are constrained by available end mill diameter/length of cut aspect ratios. Ensure that the inner surface of micro-well is as vertical and clean as possible for optimal electric field gradients and light passage through the micro-well.
Clean debris from micro-well with pressurized air. Perform another insulation test as described in 1.5.

3. Attach Electrical Leads to the Laminated Structure

Fold two 3 cm long 32 G copper wires to a right angle at 2 cm. Mix ~1.5 ml of part A and B of silver conductive epoxy.
NOTE:
Manually apply mixed silver epoxy to the top and the tips of all 3 graphene paper layers to assure good contact between layers on side A of the laminated structure (Figure 1c), then place the 1 cm copper wire end in the epoxy and between any two layers. Softly squeeze the layers to remove excess epoxy and assure good electrical contact. Repeat for side B of the laminated structure.
Place the whole device in oven rack, to dry overnight 70 °C at and 1 atm.

4. Prepare Sample and Media

Prepare isotonic media of a spectrum of conductivities using the conductivity meter, 290 mM mannitol stock solution and serial additions of isotonic phosphate buffer saline (PBS).
NOTE: A linear correlation exists between the conductivity and volume concentration of ~290 mOsm/L PBS (conducting) in ~290 mOsm/L mannitol solution (non-conducting). The video features a medium of 0.01 S/m conductivity.
Mix polystyrene beads with prepared conductivity media or e-pure water (~5 x 10^-6 S/m) to a 1:50 vol:vol ratio. This protocol is easily adaptable to biological cells as well.

5. Setup Experiment and Operate Device

Clamp the device onto a glass slide with moderate pressure (Figure 2d) using modified paper clamps or equivalent. The footings should be close enough to the micro-well to seal the laminated structure to the glass slide preventing sample leakage. The clamp should fit within the microscope stage with pressure optimized to a) prevent deformation of the laminated structure, and b) ensure the micro-well fluid does not leak. Deformation alters the well geometry and light path reducing experiment reproducibility.
Using a micro syringe or equivalent, slowly inject ~1 µl of the sample into the micro-well and avoid introducing any bubbles. Repeat injection if necessary and use care to not damage the micro-well walls with the sharp needle. Slightly overfill the micro-well and immediately slide cover glass over the micro-well to remove excess fluid, prevent evaporation, and ensure reproducible volumes for each experiment.
NOTE: A diamond tip glasscutter works well to score and crack cover glass to size.
Secure the completed laminated microdevice to the microscope stage and attach the function generator electrode wires to the two copper leads on the device. In AxioVision (Zeiss software), click button to start the camera recording in multidimensional acquisition mode. Initiate function generator signal at a fixed time period after starting the CCD camera recording to document responses with and without the electric field applied.
NOTE: Here 100 Hz to 10 MHz frequency with a 15 V_peak-peaksignal were applied and experiments were observed at 10X magnification at 1 to 200 above the glass slide surface for 2 sec without field and ~5 min with field applied. Images were digitally saved at 1 to 5 frames per sec (fps) for further analysis.
Upon experiment completion, remove the device and dismantle the clamps. Immerse both the glass slide and device in soapy water, then rinse well. Reuse devices about 30 times with consistent performance.

6. Data Analysis and Image Processing

Analyze image data with preferred software, such as ImageJ. Calculate velocity from the particle displacement between consecutive images at a given time step.
Calculate experimental DEP force and field strength based on velocity to compile trends and compare with theory²⁹.
Measure particle velocity radially in the micro-well geometry consistent with the shape of the electric field gradient. From the micro-well edge to the center, identify 8 concentric isoelectric contours (350, 300,…50, 0 µm), which results in 7 regions.
NOTE: The time for particles to traverse the 50 µm distance was used to calculate velocity. When geometric variations necessitated it, the isoelectric contours were adjusted slightly.

Representative Results

Dielectrophoretic experiments on 6 µm polystyrene beads were conducted in a 0.38 mm³ cylindrical micro-well. Results demonstrate that a 3D laminated graphene paper-based device can illustrate similar dielectrophoretic signatures as 3D metal foil laminated devices²⁰^,²¹, traditional 2D metal-electrode²⁶^,²⁷, and 2D insulator devices²⁵. In the following experiments, an 15 V_peak-peak AC signal was applied and frequency was varied from 100 Hz to 10 MHz³⁰. Qualitative DEP results are shown in Figure 3 at time 0 before field application (first column) and after 5 min (second column) in the electric field. When no electrical field was present, particles slowly sediment to the device bottom via gravity (Figures 3a and b). Figure 3c and d demonstrate typical pDEP results at 1 kHz, as indicated by particles gathering toward the micro-well edges. Figure 3e and f illustrate nDEP at 10 MHz, as indicated by focusing of particles in the center.

Figure 4a illustrates the experimental DEP responses for conductivities between 0.0001 S/m and 1.3 S/m over a frequency range from 100 Hz to 10 MHz. Negative DEP (nDEP) or positive DEP (pDEP) was typically determined by the observation of beads moving toward the center or the edges of the micro-well. However, this is complicated by recirculating flows (20-50 µm diameter) near the edges of the micro-well that happened simultaneously with DEP behavior at two regions in the conductivity-frequency space as shown by open symbols in Figure 4a. One type of recirculating flow was observed below ~10 kHz at all conductivities tested while the other type was observed at high conductivity and high frequency. The recirculating flows alter nDEP or pDEP bead motions to varying degrees. These concurrent forces are illustrated in the parameter space in Figure 4a.

Dielectrophoretic velocities are tabulated as a function of radial position using concentric counters (Figure 5a) within the micro-well. Velocity trends with position are shown in Figure 5c. As expected, the highest velocities are observed near the micro-well edge, which corresponds to the region with the highest electric field density (Figure 5b). Particles move vertically in and out of the focal plane during a 1 min recording. However, this vertical velocity magnitude is estimated to be and therefore is negligible compared to the 5~100 µm/sec concentric velocity measured. In-plane velocities range from 5 µm/sec to 36 µm/sec, which correspond to DEP mobilities of ≈ 1.07 x 10^-16 m⁴/(V⋅sec) over the electric field density regions of 5 x 10⁴ V/m to 3 x 10⁵ V/m. Velocities are consistent with those reported in 3D systems³¹^,³², 2D electrode systems³³, and DC insulator DEP systems³⁴.

Figure 1. Fabrication process for the laminated device. a) Alternately stack 6 layers of graphene paper and 5 layers of double sided tape to prevent connection between adjacent graphene layers. b) Press layers together and cut excess double-sided tape along the red dashed line. c) Drill a micro-well in the center via micro-milling as shown in Figures 2a and b. d) Adhere two copper leads to Side A and Side B with silver epoxy. e) Final fabricated device.

Figure 2. a) Computer-controlled micro-drilling machine. b) Laminated structure is immobilized on the stage with clamps. Pressurized air is used to blow debris off the end mill. c) Microdevice experiments are conducted with a microscope, CCD camera, function generator and computer for data acquisition. d) Close-up view of microdevice clamped onto a glass slide on the microscope stage. AC electrical signal from the function generator is applied to the device through the copper leads.

Figure 3. Typical dielectrophoresis responses within the 3D laminated microdevice. A 15 V_peak-peak was applied with a medium conductivity of 1.3 x 10^-4 S/m. The first column illustrates particles at experiment start with the electric field off, and the second column shows the response after 5 min. a-b) Particles sediment to the micro-well bottom; c-d) at 1 kHz, particles gathered near the micro-well edge, indicating pDEP. e-f) At 10 MHz, particles focused to the micro-well center, indicating nDEP.

Figure 4. a) Experimental DEP behavior of 6.08 µm polystyrene beads as a function of conductivity (0.0001-1.3 S/m) and frequency (100 Hz- 10 MHz) in PBS adjusted mannitol solution. Small recirculations were observed concurrent with DEP behavior near micro-well edges for low frequencies (<1 kHz) and all tested medium conductivities, as well as at high frequencies and higher medium conductivity. Open symbols and represent negative DEP and positive DEP with recirculation, while the solid symbol represents nDEP without recirculation. Below ~100 Hz, electrolysis bubbles were observed and are represented by Δ. b) Predicted crossover frequencies from 0.0001 S/m to 1.3 S/m. Please click here to view a larger version of this figure.

Figure 5. a) Images of polystyrene beads experiencing nDEP in a 15 V_peak-peak, 1MHz field. Concentric circles track the movement of particles as they traverse the microwell. b) COMSOL simulation of electric field gradient (V/m²) of a cross-section of micro-well. c) Dielectrophoretic velocities of clusters of beads as a function of radial position within the micro-well. Please click here to view a larger version of this figure.

Discussion

This manuscript details protocols for fabricating a novel 6 graphene layer and 5 tape layer microdevice. Further, device operation is illustrated via observed DEP behaviors of 6.08 µm polystyrene beads along with a unique, geometrically relevant particle velocity analysis approach. This versatile approach to construct nonlinear electrokinetic devices is less costly than electrode and fluidic layer microfabrication techniques, while yielding equally reliable results.

Further, this novel 3D graphene paper microdevice yielded experimental dielectrophoretic results that agree with both the theoretically predicted behavior and previously reported experimental results³⁵. For signal frequencies from 100 Hz up to 10 MHz and media conductivities from 1 x 10^-4 S/m to 1.3 S/m, experiments verified the existence of a crossover frequency, below which pDEP was observed and above which nDEP was observed. As expected, nDEP was observed over most of the conductivity-frequency space as shown in Figure 4a. Theory predicts that 6.08 µm homogeneous polystyrene beads ( ε_ρ = 2.55, σ_ρ = 1.3 x 10^-3 S/m ³⁶) have a crossover frequency when σ_m < σ_ρ = 1.3 x 10^-3 S/m. In the remaining parameter space from 1 x 10^-4 S/m to approximately 1.3 x 10^-3 S/m, crossover frequencies (f_co on the order of ≈ 1 kHz were observed. For example, f_co was 1 kHz in a 3.9 x 10^-4 S/m medium, while a previous reported experimental result at 1.0 x 10^-3 S/m was f_co = 5 kHz ³⁵, and model-based predicted value was 168 kHz ^37-39. These three results are considered in rough agreement given the sensitivity of crossover frequency to change in medium conductivity in the specific region⁴⁰, as well as other compounding charge induced factors and equipment variations. As shown in Figure 4b, as media conductivity changes slightly from 1 x 10⁴ S/m to 1.3 x 10^-3 S/m, corresponding crossover frequencies decrease 2 orders of magnitude or more. Using 168 kHz as the crossover frequency in the model, with parameters fixed as specified above, one can solve for the particle conductivity and find it to be 1.00 x 10^-3 S/m compared to the real value of 1.3 x 10^-3 S/m (23% difference).

Observations of two types of recirculating flows in the conductivity-frequency space were observed and attributed to AC electroosmosis and electrothermal flows in the low and high frequency regions, respectively. For low frequencies (<10 kHz) at all tested medium conductivities, local particle recirculation velocities increased as frequency decreased with slight changes due to medium conductivity. Both the conductivity-frequency conditions and size of the recirculating rolls (20-50 µm) agree with previous AC electroosmotic flow studies^41-43. For relative high frequencies (>100 kHz) at relative high medium conductivity (>0.01 S/m), nDEP begins to be overpowered by recirculation. Recirculating particle velocities increased as the medium conductivity increased and frequency increased. Again, both the conductivity-frequency conditions and the size of recirculations agree with previous findings^44-47.

In 3D DEP, particles also experience dielectrophoretic forces pushing particles between adjacent graphene paper layers at multiple vertical positions in the micro-well. The optical microscope observation of this is partially compromised because light is scattered by focused DEP particles above and below the plane of interest. Due to gravity sedimentation over time, more particles were observed near the bottom DEP focusing planes than in the upper-most DEP focusing planes (data not shown)⁴⁸.

Device fabrication is extremely versatile; the protocols provided can be easily adapted for devices with more layers or other materials. As an alternative insulation layer material, polydimethylsiloxane (PDMS) thin films can be spincoated to a controllable and fairly uniform thickness. PDMS has well characterized electrical and surface chemistry properties, but handling such thin fragile films was troublesome. Double-sided tape has more uniform thickness, was easier to handle with better layer-to-layer sealing and thus yielded higher success rate of optimally functional devices. The XG Sciences graphene paper (Leaf B-072) functioned well as an electrode material and tailored manufacturing offered versatile electrical and mechanical qualities. Higher nanoplatelet concentrations reduced paper resistivity²⁴ and polymeric supports prevented water adsorption while cellulosic supports allow water diffusion (data not shown).

Complications with device functionality can include increased resistivity at the well surface, broken electrical connections, electrolytic bubbles, bubble introduction during sample loading, and skewed well geometry. The insulation test in procedure step 1.5 should be utilized prior to each experiment to assess device integrity. The utilized XG graphene paper surface exposed to the microwell wore off after ~30 experiments. Inconsistent DEP results were easily recognized via erratic global flow through the micro-well or no response to an applied potential. Side A and Side B (Figure 1c) graphene layers of the device may break if not handled gently. In these cases, replacement devices are needed. At frequencies at or below 100 Hz, the 3D graphene electrodes catalyzed water electrolysis to produce O₂ and H₂ bubbles. This frequency threshold is 2 orders of magnitude lower than this group’s previous results with traditional microfabricated 2D electrodes⁴⁹, which expands the operating space in which particles or biological cells can be interrogated. Air bubbles from the sample syringe should be avoided due to electric field shape and optical interferences. Lastly, perfectly vertical micro-well drilling is critical for consistent optical illumination and observation of DEP behaviors. Micro-well skewing becomes more difficult to manage as the number of laminated layers increases. Most confocal and light microscopes have working distances below 1 mm, so DEP behaviors cannot be easily observed at thickness above this. However, increasing the third dimension would be advantageous for large scale DEP processing.

A straightforward laminated graphene paper/tape structure has been demonstrated as a batch-wise 3D DEP microdevice. In future applications, particle or cell suspension could continuously flow through the device to achieve higher-throughput DEP sorting⁵⁰. Specific biomedical applications that require sorting of large volumes to separate and identify rare cells include detection of circulating tumor cells⁵¹ and sepsis⁵². In addition, water-absorbing graphene paper could simultaneously function as an electrode and diffusion medium for particle/cell concentrators. Lastly, graphene paper has been demonstrated as a viable biosensor²⁴. The device described here could be used for simultaneous DEP concentration and biological detection at the graphene surface. Thus, different graphene paper types may be useful electrodes in high throughput microfluidic systems employing electrokinetics and/or biosensors.

Disclosures

The authors have nothing to disclose.

Acknowledgements

Thanks to XG Sciences for generous donations of graphene paper. Thanks to Dr. C. Friedrich for generously letting us use the micro-drilling equipment. A special thanks is extended to Tayloria Adams for narrating the video.

Materials

Reagents
Name of Reagent	Company	Catalogue Number	Comments
Polystyrene Beads	Spherotech, Inc.	PP-60-10	6.08 um diameter
Graphene paper	XG Sciences, Inc.	XG Leaf B-072
Double sided tape	3M	N/A	136 office tape
Silver conductive epoxy	MG chemicals	8331-14G	Part A &B included
Mannitol	Sigma Aldrich	091M0020V
Phosphate buffer saline	OmniPur	0381C490

Equipment:
Name of equipment	Company	Catalogue Number	Comments
Microscope (CCD Camera)	Zeiss	Axiovert 200M
Function/waveform generator	Agilent	33250A
Syringe	Hamilton	84505
Paper Clamp	ADAMS	3300-50-3848
Oven	Fisher Scientific	280A
Multimeter	OMEGA	HHM25
Micro-milling machine	AEROTECH	ABL1500 stages/A3200 Npaq controller
End mill	ULTRATOOL	708473
AxioVision	Zeiss	Version4.8

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Xie, H., Tewari, R., Fukushima, H., Narendra, J., Heldt, C., King, J., Minerick, A. R. Development of a 3D Graphene Electrode Dielectrophoretic Device. J. Vis. Exp. (88), e51696, doi:10.3791/51696 (2014).