Source: Lee, C., et al. Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection. J. Vis. Exp. (2020).
This video demonstrates a technique for the detection of extrapulmonary tuberculosis. Superparamagnetic iron oxide (SPIO) nanoprobes, conjugated to Mycobacterium-specific antibodies, are injected into a mouse previously infected with Mycobacterium bovis BCG, labeling the granuloma and providing a better imaging contrast for its detection.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. SPIO-MtbsAb synthesis
2. Particle morphology observation and relaxation tier measurement
3. Cell imaging
4. BCG (Bacillus Calmette–Guérin) inoculation
5. In vivo MRI
The authors have nothing to disclose.
(benzotriazol-1-yloxy) tripyrrolidinophosphonium hexafluorophosphate | Sigma-Aldrich | ||
1-hydroxybenzotriazole | Sigma-Aldrich | ||
epichlorohydrin, 2,2'-(ethylenedioxy)bis(ethylamine) | Sigma-Aldrich | ||
Human monocytic THP-1 | |||
M. bovis BCG | Pasteur Mérieux | Connaught strain; ImmuCyst Aventis | |
MRI | GE medical Systems | 3.0-T, Signa | |
NMR relaxometer | Bruker | NMS-120 Minispec | |
SPECTRUM molecular porous membrane tubing, 12,000 -14,000 MW cut off | Spectrum Laboratories Inc | ||
TB surface antibody- Polyclonal Antibody to Mtb | Acris Antibodies GmbH | BP2027 | |
transmission electron microscope | JEOL | JEM-2000 EX II |