Source: Du, Y., et al. Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model. J. Vis. Exp. (2018)
This video demonstrates the hematoxylin and eosin (H&E) staining method to evaluate colon damage in a murine model of DSS-induced colitis — an inflammatory bowel disease. Upon isolating the colon from a DSS-treated mouse and obtaining sections of the tissue, H&E staining of the sections helps identify the inflammation-mediated tissue damage.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of Mice and DSS
2. Induction and Evaluation of DSS Colitis in Mice
3. Preparation of Tissue Samples
4. Histological Assessment of the Severity of DSS-induced Colitis
The authors have nothing to disclose.
Dextran Sulfate Sodium Salt (DSS) | MP Biomedicals | 2160110 | |
H&E Staining Kit | BBI Life Sciences | E607318 | |
Phosphate-Buffered Saline (PBS) | Sangon Biotech | B548117 | |
BD 10 ml Syringe | BD Biosciences | 309604 | |
Instruments and equipment | |||
Balance | |||
Scissors | |||
Forceps | |||
Staining jars | |||
Software | |||
Imag-Pro Plus | Media Cybernetics, Inc. |