This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Laparotomy
Anesthetize animals with xylazine (10 mg/kg) and ketamine solution (80 mg/kg), subcutaneously (0.1 mL/10g of body weight) using a 1 mL syringe and 13 x 0.45 mm needle 26 G ½. Check for sufficient anesthesia depth by pinching the toe. Control body temperature using heated pads. Ensure that all surgical materials are sterile.
Clean the abdominal area with 5% povidone-iodine solution and use a trimmer to remove hair between the chest and lower abdomen (approximately 2 cm2). Clean the surgical area with 70% alcohol.
Immobilize the animal on the surgical board using surgical tape. Use scissors to cut 5 mm of the skin horizontally, on the upper part of the abdomen and 1 cm below the xiphoid process. Repeat the cut on the peritoneum. This will result in a laparotomy with minimal exposure of the cavity.
2. Locating and Exposing the Pancreas
With the aid of a retractor, pull the liver towards the mouse's head, ~1 cm from the intestine.
Locate the region of the pancreas that will be injected with sodium taurocholate (pancreas head). Locate the duodenum with reference to the liver—below the liver, on the right side (on the left as the mouse is viewed). The duodenum is the first part of the small intestine and is connected to the final portion of the stomach.
With the aid of forceps, lift the liver towards the animal head, and gently pull the small intestine portion. Fix the two lateral ends of the small intestine with a 6-0 polypropylene suture to better view the distal portion of the common bile duct.
3. Severe Acute Pancreatitis Induction
Temporarily occlude the proximal common bile duct with a microvessel clip to prevent retrograde infusion from leaking into the liver. The common bile duct can be seen on the liver side of the duodenum and its junction with the duodenum will appear white. Expose the organ out of the abdominal cavity.
Puncture the periampullary region (whitish part of the small intestine's wall) to access the common bile duct with a 0.4 mm needle connected to a 0.54 mm polyethylene tube.
Make a temporary occlusion of the distal common bile duct with 8-0 suture to prevent the sodium taurocholate solution from leaking into the duodenum.
Start the infusion pump and program a 2.5% sodium taurocholate solution (diluted in 0.9% saline) infusion at a constant speed of 10 µL/10 g body weight for 3 min.
After the infusion, remove the microvessel clip, the temporary 8-0 suture, and the injection needle from the bile pancreatic duct to reconstitute the physiological flow of the bile.
At the end, suture the abdomen with 6-0 nonabsorbent monofilament polypropylene suture. The time between the laparotomy and the end suture should be a maximum of 30 min (see Figure 1).
After the surgery, house the animals in polyethylene boxes lined with wood shavings and water and food ad libitum.
Treat control mice in the same manner as the experimental mice but ensure that the infusate consists of saline only. Perform the surgical procedure and the infusion of saline solution (10 mL/min, for 3 min) in a control group (SHAM) to eliminate the inflammatory bias caused by surgery and cannulation.
Use tramadol 12.5 mg/kg subcutaneously every 8 hours, starting after post-surgical recovery.
Representative Results
Figure 1:Schematics of severe acute pancreatitis induction by 2.5% sodium taurocholate in C57BL/6 mice.(A) gallbladder; (B) common bile duct; (C) pancreatic duct; (D) portal vein; (E) microvessel clip; (F) puncture site (needle attached to a polyethylene tube and connected to infusion pump); (G) temporary needle fixation in the common bile duct.
Disclosures
The authors have nothing to disclose.
Materials
0.4 mm needle
INTRAG MEDICAL TECH
90183210
30G
0.54 mm polyethylene tube
Tygon
730010
Styrofoam block
Masking tape for mounting the mouse
Missner
1236
Infusion pump scheduled to 10µL/min.
Havard aparatus-Peristaltic Pump Series
MA1 55-7766
Model 66 Small Peristaltic
Scissors and forceps
Antiseptic providine iodine
Pfizer
12086OR
Antisepsis
70% ethanol
SIGMA
459836
Mix 700 mL 100% ethanol with 300 mL dH2O
Razor blade
Lord
bdk9a1ghk6
For trichotomy
Sodium taurocholate
Sigma-Aldrich
86339- 1G
CAS NUMBER- 345909-26-4
Microvessel clip
Medicon Surgical
56.87.35
Approximator, opening 4.0 mm, closing pressure 30-40 g
Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion. J. Vis. Exp. (Pending Publication), e20750, doi: (2023).