Ultrasound-Guided Implantation: A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse

Published: April 30, 2023

Abstract

Source: Hay, C. A. et al.  Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma. J. Vis. Exp. (2019).

This video describes the protocol for ultrasound-guided orthotopic implantation of murine-derived pancreatic ductal adenocarcinoma cells directly into the pancreas of the mouse. This causes less inflammation at the implantation site compared to surgical procedures.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the jove veterinary review board. 1. Pre-Surgical Preparation of Mice NOTE: This step is recommended to be performed 24 hours prior to the procedure. Place cages containing experimental mice on a warmer set to 37°C. Obtain new, clean cages and place on a second warming platform set to 37°C.</…

Representative Results

Figure 1: Ultrasound-guided implantation of PDA cells into the murine pancreas. (A) Orientation of mouse, ultrasound stage, and ultrasound probe used to obtain a high-resolution image of abdominal organs. Note the stage and platform have been turned 90° from the standard orientation to allow easy access to the upper left quadrant of mouse abdomen. (B) Ultrasonographic image …

Disclosures

The authors have nothing to disclose.

Materials

50 mL Conicals Thomas Scientific 2602A26
Blunt edged forceps Fine Science Tools 11000-12
Cell Dissociation Buffer Thermo-Fisher 13151014
Cotton Tipped swabs Thermo-Fisher 19062614
Covidien Monoject 3/10mL, 29G X 1/2" Thermo-Fisher 8881600145
Depilatory Agent Amazon Nair Body Lotion
Forceps (blunt edge) Fine Science Tools 11000-12
Gauze Fisher 13-761-52
Gentamicin Thermo-Fisher 15750060
Induction Chamber VetEquip 941444
Isofluorane Penn Vet Supply VED1350
Isofluorane Vaporizer VetEquip 911103
L-glutamine Thermo-Fisher 25030081
Optixcare MidWest Veterinary Supply 052.50310.3
Paper Tape Medline MMM1530Z5
PBS Thermo-Fisher 14-190-250
Slide warmer C&A Scientific XH-2001
Sterilant (Clidox-S) Fisher Scientific NC0332382 (activator) NC9189926 (base) Needs to be combined according to manufacturer's instructions
Sterile Alcohol prep pad Covidien 6818
Trypsin Thermo-Fisher 15090046
Ultrasound gel Thermo-Fisher 03-34-1LT
Visualsonics Ultrasound Vevo 2100 Visual Sonics Vevo 2100

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Cite This Article
Ultrasound-Guided Implantation: A Technique to Implant Pancreatic Ductal Adenocarcinoma Cells into an Adult Mouse. J. Vis. Exp. (Pending Publication), e20307, doi: (2023).

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