Centrifugation is a method of separating suspended particles from a liquid by spinning in a centrifuge at a high number of revolutions per minute. The particles sediment at a rate proportional to the centrifugal force they experience. After centrifugation, the particles settle at the bottom, while the supernatant liquid can be decanted. When the components are of equal density, the heavier particles sediment first. However, among particles with equal mass, the denser particles sediment first. In differential centrifugation, heavier particles are eliminated first at lower speeds. The centrifugation speed is increased stepwise until the target particles are isolated. In density gradient centrifugation, the density of the solution increases toward the bottom of the tube. Upon centrifugation, each component stops moving when it reaches a layer with equal density, resulting in separate bands.