To begin the experiment, collect five two-week old grown plants from the generation one population that appear healthy. Note: Plants should appear green, not wilted, and not have damaged leaves. Experimental Hypothesis: The experimental hypothesis for this lab might be that the number of lead trichomes varies naturally, but the average number will increase over generations if only plants with the highest number of trichomes are interbred or decrease if those with the least are interbred. Null Hypothesis: The null hypothesis might be that the average number of trichomes will not change over subsequent generations after artificial selection.
Label each plant with a letter from A to E and with both partners’ names.
Record any natural differences you see between your plant samples. Note: All of the plants are the same age, so any observed variation is due to expressed phenotype, not development.
Use a hand lens to count the number of trichomes found on the petiole of the lowest leaf, not including the round, first-grown cotyledons for each plant. Note: It can be helpful to shine a light onto the plant and to hold it against a dark background, such as a lab bench, during the trichome analysis.
Both partners will conduct counts on each plant independently and compare answers. If the counts are different, enlist a third counter or take the average of the two results.
Determine the top and bottom 10% of the plants from the class trichome count. These groups will make up the two artificially selected lines: high hairiness and low hairiness, respectively.
If more than 10% of the plants have no trichomes, select 10% of those plants randomly for the low hairiness plant line.
Separate the two groups of selected plants and label them clearly.
Move the plants to a well-lit area and keep them well watered until they develop flowers. Note: Flowers typically develop around 14 days.
When the flowers are fully developed, use a pollination wand or paintbrush to rub the stigma and anther of one plant’s flowers for a few seconds.
Using the same wand or brush, rub the inside of each flower of every other plant in the same selection line.
Using a different wand or brush, repeat steps 11 through 12 on the other line. Note: Take care not to cross the lines. Spread the pollen only among the appropriately selected plants.
Return the generation one plants to the growing areas. When the plants reach approximately 1 month old, they should produce mature seed pods.
Allow the plants to dry out once the seed pods turn yellow.
Break the seed pods open and collect the seeds.
Obtain new pots for the generation two seeds.
Fill the pots with moist potting soil about halfway and add a fertilizer pellet to each pot.
Fill the pots with more soil to fill just below the lip.
Make a shallow depression in the soil of each pot with a probe.
Touch a clean toothpick to a gluestick and use the toothpick to transplant the seeds into the soil one by one.
Cover the seeds with a thin layer of soil.
Label the pots with their hairiness line and place them in the designated growing area.
Use a plastic dropper to water the plants as needed to ensure they do not dry out.
When the plants are two weeks old, collect five plants from each line and label them A to E.
