JOVE JOURNAL

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Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Madison T. Jones, Samantha W. Manioci, Ashley E. Russell²

¹Department of Biology, School of Science,Penn State Erie, The Behrend College, ²Magee Womens Research Institute—Allied Member

Journal (Neuroscience)

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

María V. Vaglienti², Paula V. Subirada³, Pablo F. Barcelona², Gustavo Bonacci², María C. Sanchez²

¹Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas,Universidad Nacional de Córdoba, ²Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI),Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), ³Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC),Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

Journal (Neuroscience)

A Stably Established Two-Point Injection of Lysophosphatidylcholine-Induced Focal Demyelination Model in Mice

Xiao-Wei Pang, Man Chen, Yun-Hui Chu, Yue Tang, Chuan Qin, Dai-Shi Tian

¹Department of Neurology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology

Journal (Neuroscience)

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Kenta Yamauchi², Shinichiro Okamoto^2,3, Megumu Takahashi^2,4,5, Masato Koike³, Takahiro Furuta, Hiroyuki Hioki^2,7

¹Department of Neuroanatomy,Juntendo University Graduate School of Medicine, ²Department of Cell Biology and Neuroscience,Juntendo University Graduate School of Medicine, ³Advanced Research Institute for Health Sciences,Juntendo University, ⁴Department of Neuroscience, Graduate School of Medicine,Kyoto University, ⁵Japan Society for the Promotion of Science, ⁶Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry,Osaka University, ⁷Department of Multi-Scale Brain Structure Imaging,Juntendo University Graduate School of Medicine

Journal (Neuroscience)

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Mohammed R. Shaker*¹, Zoe L. Hunter*¹, Ernst J. Wolvetang

¹Australian Institute for Bioengineering and Nanotechnology,The University of Queensland

Journal (Neuroscience)

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Arun Upadhyay, Robert J. Vassar, Jeffrey N. Savas

¹Department of Neurology,Northwestern University Feinberg School of Medicine

Journal (Neuroscience)

Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae

Friederike Steenken, Sonny Bovee, Christine Köppl

¹Cluster of Excellence "Hearing4all" and Research Centre Neurosensory Science, Department of Neuroscience, School of Medicine and Health Science,Carl von Ossietzky University Oldenburg

Journal (Neuroscience)

In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility

Claire W. Chen, Anna M. Potenski, Crystal K. Colón Ortiz, Maria I. Avrutsky*¹, Carol M. Troy*^1,3,4

¹Department of Pathology & Cell Biology; Vagelos College of Physicians and Surgeons,Columbia University, ²Department of Molecular Pharmacology and Therapeutics; Vagelos College of Physicians and Surgeons,Columbia University, ³Department of Neurology; Vagelos College of Physicians and Surgeons,Columbia University, ⁴The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain; Vagelos College of Physicians and Surgeons,Columbia University

Journal (Neuroscience)

Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections

Alex R. Eaker, Katherine T. Baldwin²

¹Neuroscience Center,University of North Carolina, Chapel Hill, ²Department of Cell Biology and Physiology,University of North Carolina, Chapel Hill

Journal (Neuroscience)

Employing Transcranial Magnetic Stimulation in a Resource Limited Environment to Establish Brain-Behavior Relationships

Taylor Shelansky, Katherine Chavarria, Kitty Pagano, Sarah Sierra, Vanessa Martinez, Nathira Ahmad, Janet Brenya, Aleksandra Janowska, Samantha Zorns, Abigail Straus, Victoria Mistretta, Brianna Balugas, Matthew Pardillo, Julian Paul Keenan

¹The Cognitive Neuroimaging Laboratory,Montclair State University

Journal (Neuroscience)

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Belquis Nassim-Assir, Daniel Ouro Corredera, Rodrigo Alvarez-Pardo, Susanne tom Dieck, Elena Ciirdaeva, Erin M. Schuman, Beatriz Alvarez-Castelao

¹Max-Planck-Institute for Brain Research,Synaptic Plasticity Department, ²Department of Biochemistry and Molecular Biology,Veterinary School, Complutense University of Madrid

Journal (Neuroscience)

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique

Matheus H. Tempone, Hercules R. Freitas, Clarissa S. Schitine, Ricardo A. de Melo Reis

¹Laboratory of Neurochemistry, Institute of Biophysics Carlos Chagas Filho,Universidade Federal do Rio de Janeiro, ²Laboratory of Neuroenergetics and Inborn Errors of Metabolism, Institute of Medical Biochemistry Leopoldo de Meis,Universidade Federal do Rio de Janeiro, ³Laboratory of Neurochemistry and Cell Biology, Institute of Life Sciences,Universidade Federal da Bahia

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