JOVE JOURNAL

면역학 및 감염병학

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Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Camila Gachet-Castro*¹, Lays Adrianne Mendonça Trajano-Silva*¹, Munira Muhammad Abdel Baqui¹

¹Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School,University of Sao Paulo, Ribeirao Preto, SP, Brazil

Journal (Immunology and Infection)

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Katharine M. Ng^1,2, Carolina Tropini^1,2,3

¹School of Biomedical Engineering,University of British Columbia, ²Department of Microbiology and Immunology,University of British Columbia, ³Humans and the Microbiome Program,Canadian Institute for Advanced Research (CIFAR)

Journal (Immunology and Infection)

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

Shalhevet Azriel*^1,2, Hadar Bootz*^1,2, Alon Shemesh*^1,2, Sivan Amidror^1,2, Nissan Yissachar^1,2

¹The Goodman Faculty of Life Sciences,Bar-Ilan University, ²Bar-Ilan Institute of Nanotechnology and Advanced Materials,Bar-Ilan University

Journal (Immunology and Infection)

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Amira D. Rghei¹, Brenna A. Y. Stevens*¹, Sylvia P. Thomas*¹, Jacob G. E. Yates*¹, Benjamin M. McLeod¹, Khalil Karimi¹, Leonardo Susta¹, Byram W. Bridle¹, Sarah K. Wootton¹

¹Department of Pathobiology,University of Guelph

Journal (Immunology and Infection)

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Mario Wenger¹, Athanasios Bethanis¹, Litty Johnson¹, Lorenz Aglas¹

¹Department of Biosciences,University of Salzburg

Journal (Immunology and Infection)

Assessing Respiratory Immune Responses to Haemophilus Influenzae

Lovisa Dousha^1,2, Roleen Sharma^1,2, Steven Lim^2,3, James Ngui^2,4, Ashley M. Buckle⁵, Paul T. King^1,2

¹Monash Lung and Sleep,Monash Medical Centre, ²Monash University Department of Medicine,Monash Medical Centre, ³Flow Cytometry Science Technology Platform,Francis Crick Institute, ⁴Clinical Immunology,Monash Medical Centre, ⁵Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute,Monash University

Journal (Immunology and Infection)

Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

Noémie Luyts¹, Greetje Vande Velde², Matthias Vanneste¹, Helene De Bruyn¹, Annelies Janssens¹, Natalie Verstraeten³, Thomas Voets¹, Wouter Everaerts⁴

¹Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine,KU Leuven, ²Biomedical MRI, Department of Imaging & Pathology,KU Leuven, ³Belgium & KU Leuven Centre of Microbial and Plant Genetics,VIB-KU Leuven Center for Microbiology, Leuven, ⁴Laboratory of Experimental Urology, Department of Development and Regeneration,KU Leuven

Journal (Immunology and Infection)

Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)

Andrew Lees¹, James Zhou¹

¹Fina Biosolutions LLC

Journal (Immunology and Infection)

Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Paula Ordonez¹, Kate N. Bishop², Jonathan P. Stoye¹, Harriet Cordelia Theed Groom³

¹Retrovirus-Host Interactions Laboratory,The Francis Crick Institute, ²Retroviral Replication Laboratory,The Francis Crick Institute, ³Sidney Sussex College, Department of Medicine,University of Cambridge

Journal (Immunology and Infection)

Partial Heterotopic Hindlimb Transplantation Model in Rats

Marion Goutard^1,2,3, Mark A. Randolph^1,2,3, Corentin B. Taveau^1,2,3,4, Elise Lupon^1,2,3, Laurent Lantieri⁴, Korkut Uygun^1,2,3, Curtis L. Cetrulo Jr.*^1,2,3, Alexandre G. Lellouch*^1,2,3,4

¹Division of Plastic and Reconstructive Surgery,Massachusetts General Hospital, Harvard Medical School, ²Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences,Massachusetts General Hospital, Harvard Medical School, ³Shriners Hospital for Children, ⁴Service de Chirurgie Plastique, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris (APHP),Université de Paris

Journal (Immunology and Infection)

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Taylor R. Jamieson^1,2, Joanna Poutou^1,2, Ricardo Marius^1,2, Xiaohong He¹, Reza Rezaei^1,2, Taha Azad^1,2, Carolina S. Ilkow^1,2

¹Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology,University of Ottawa

Journal (Immunology and Infection)

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Eunbee Park¹, Megan A. Evans⁴, Heather Doviak⁴, Keita Horitani⁴, Hayato Ogawa⁴, Yoshimitsu Yura⁴, Ying Wang², Soichi Sano^3,4, Kenneth Walsh^1,4

¹Department of Biochemistry and Molecular Genetics,University of Virginia School of Medicine, ²Department of Cardiology, Xinqiao Hospital,Army Medical University, ³Department of Cardiology,Osaka City University Graduate School of Medicine, ⁴Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine

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