JOVE JOURNAL

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Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Aneta Babula*^1,2, Izabela Siemińska*^1,3, Monika Baj-Krzyworzeka

¹Department of Clinical Immunology,Jagiellonian University Medical College, ²Doctoral School of Medical and Health Sciences,Jagiellonian University, ³Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Journal (Immunology and Infection)

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Huizheng Ge*¹, Linlin Zhang*^2,3, Mengjia Liu*^2,3, Xi Yang, Xu Wu, Ran Wang³, Zhengde Xie³

¹Center of Excellence (Beijing),Becton Dickinson Medical Devices (Shanghai) Co Ltd, ²Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital,Capital Medical University, National Center for Children's Health, ³Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences

Journal (Immunology and Infection)

Gene Editing of Primary Rhesus Macaque B Cells

Harald Hartweger, Rajeev Gautam, Yoshiaki Nishimura, Fabian Schmidt⁵, Kai-Hui Yao, Amelia Escolano⁶, Mila Jankovic, Malcolm A. Martin, Michel C. Nussenzweig⁴

¹Laboratory of Molecular Immunology,The Rockefeller University, ²Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, ³Laboratory of Retrovirology,The Rockefeller University, ⁴Howard Hughes Medical Institute,The Rockefeller University, ⁵Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), ⁶Vaccine and Immunotherapy Center,Wistar Institute

Journal (Immunology and Infection)

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

Xinrui Wang², Ruizhong Zhang, Zefeng Lin, Ming Fu, Yan Chen²

¹School of Medicine,South China University of Technology, ²Provincial Key Laboratory of Research in Structure Birth Defect Disease and Department of Pediatric Surgery,Guangzhou Women and Children’s Medical Center

Journal (Immunology and Infection)

The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model

Yanis Berkane*^1,2,3,5, Austin Alana Shamlou*^1,2,4,5, Jose Reyes², Hyshem H. Lancia^2,5, Irina Filz von Reiterdank^2,5,6,7, Nicolas Bertheuil, Basak E. Uygun^5,6, Korkut Uygun^2,5,6, William G. Austen Jr.⁴, Curtis L. Cetrulo Jr.^2,4,5, Mark A. Randolph^2,4,5, Alexandre G. Lellouch^2,5,6,8

¹Vascularized Composite Allotransplantation Laboratory,Massachusetts General Hospital, ²Harvard Medical School, ³Department of Plastic, Reconstructive and Aesthetic Surgery, Rennes University Hospital Center (CHU de Rennes),University of Rennes 1, ⁴Plastic Surgery Research Laboratory,Massachusetts General Hospital, ⁵Shriners Children's Boston, ⁶Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, ⁷Department of Plastic Surgery and Hand Surgery,University Medical Center Utrecht, ⁸Department of Plastic, Reconstructive, and Aesthetic Surgery, Groupe Almaviva Santé,Clinique de l'Alma

Journal (Immunology and Infection)

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14⁺ Monocytes

Patricia Riedlova*¹, Shatakshi Sood*¹, Carl S. Goodyear, Cecilia Ansalone

¹School of Infection & Immunity,University of Glasgow

Journal (Immunology and Infection)

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Joo-Hui Han, Rebecca E. Tweedell, Thirumala-Devi Kanneganti

¹Department of Immunology,St. Jude Children's Research Hospital

Journal (Immunology and Infection)

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Tiffany Snow, Jon Roussey, Casey Wegner, Brandon McNaughton

¹Research and Development,Akadeum Life Sciences, ²Akadeum Life Sciences

Journal (Immunology and Infection)

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Loni Perrenoud, James Conley, Leslie J. Berg

¹Department of Immunology and Microbiology,University of Colorado-Anschutz School of Medicine

Journal (Immunology and Infection)

Establishment of Deep Hypothermic Circulatory Arrest in Rats

Weidong Yan, Bingyang Ji

¹Department of Cardiopulmonary Bypass, National Center for Cardiovascular Disease and Fuwai Hospital, State Key Laboratory of Cardiovascular Medicine, Chinese Academy of Medical Science,Peking Union Medical College

Journal (Immunology and Infection)

A Rapid, Simple, and Standardized Homogenization Method to Prepare Antigen/Adjuvant Emulsions for Inducing Experimental Autoimmune Encephalomyelitis

B. Thomas Bäckström²

¹The Rausing Laboratory, Autoimmunity Section, Division of Neurosurgery, Department of Clinical Sciences,Lund University, ²Department of Autoimmunity,BTB Emulsions

Journal (Immunology and Infection)

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Xing Luo, Yanan Tong, Xiaoqiang Zeng, Yan Ye, Yun Yang, Zhen Song, Zelong Zhang, Haibo Li, Jining Gao, Xuhu Mao, Hao Zeng, Quanming Zou, Hongwu Sun

¹Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy,Third Military Medical University of Chinese PLA, ²Institute of Combined Injury of PLA, College of Military Preventive Medicine,Third Military Medical University of Chinese PLA

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