JOVE JOURNAL

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Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Naemeh Pourshafie¹, Philip R. Lee², Ke-lian Chen¹, George G. Harmison¹, Laura C. Bott^1,3, Kenneth H. Fischbeck¹, Carlo Rinaldi^1,4

¹Neurogenetics Branch, National Institute of Neurological Disorders and Stroke,National Institutes of Health, ²Section on Nervous System Development and Plasticity, The Eunice Kennedy Shriver National Institute of Child and Human Development,National Institutes of Health, ³Department of Molecular Biosciences, Rice Institute for Biomedical Research,Northwestern University, ⁴Department of Physiology, Anatomy and Genetics,University of Oxford

Journal (Genetics)

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Wing H. Wong*^1,2, R. Spencer Tong*^1,2, Andrew L. Young^1,2, Todd E. Druley^1,2

¹Department of Pediatrics, Division of Hematology and Oncology,Washington University School of Medicine, ²Center for Genome Sciences and Systems Biology,Washington University School of Medicine

Journal (Genetics)

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

Adam C. Silver¹

¹Department of Biology,University of Hartford

Journal (Genetics)

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Yuki Yoshida^1,2, Sayuri Konno^1,3, Ryousuke Nishino^1,3, Yumi Murai^1,3, Masaru Tomita^1,2,3, Kazuharu Arakawa^1,2,3

¹Institute for Advanced Biosciences,Keio University, ²Systems Biology Program, Graduate School of Media and Governance,Keio University, ³Faculty of Environment and Information Studies,Keio University

Journal (Genetics)

Mating-based Overexpression Library Screening in Yeast

Elliott Hayden¹, Shuzhen Chen¹, Abagail Chumley¹, Quan Zhong¹, Shulin Ju¹

¹Department of Biological Sciences,Wright State University

Journal (Genetics)

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

Ceres Kosuta^1,2, Kate Daniel¹, Devon L. Johnstone^1,2, Kevin Mongeon^1,2, Kevin Ban^1,2, Sophie LeBlanc¹, Stuart MacLeod¹, Karim Et-Tahiry², Marc Ekker², Alex MacKenzie¹, Izabella Pena^1,2

¹Children's Hospital of Eastern Ontario Research Institute, ²Department of Biology,University of Ottawa

Journal (Genetics)

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Adam B. Cornwell¹, Jesse R. Llop¹, Peter Salzman^2,3, Juilee Thakar⁴, Andrew V. Samuelson¹

¹Department of Biomedical Genetics,University of Rochester Medical Center, ²Department of Biostatistics and Computational Biology,University of Rochester Medical Center, ³Non-Clinical Statistics,Bristol-Myers Squibb, ⁴Department of Microbiology and Immunology,University of Rochester Medical Center

Journal (Genetics)

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Venkatramanan Krishnamani¹, Tabitha A. Peterson¹, Robert C. Piper¹, Mark A. Stamnes¹

¹Molecular Physiology and Biophysics,University of Iowa

Journal (Genetics)

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Stefan Schoenfelder*¹, Biola-Maria Javierre*^1,2, Mayra Furlan-Magaril^1,3, Steven W. Wingett^1,4, Peter Fraser^1,5

¹Nuclear Dynamics Programme, The Babraham Institute,Babraham Research Campus, ²IJC Building, Campus ICO-Germans Trias i Pujol,Josep Carreras Leukemia Research Institute, ³Departamento de Genética Molecular, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México, ⁴Bioinformatics Group, The Babraham Institute,Babraham Research Campus, ⁵Department of Biological Science,Florida State University

Journal (Genetics)

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Sarah E. Ashley^1,2, Braydon A. Meyer^2,3, Justine A. Ellis^2,3,4, David J. Martino^2,3,5

¹Molecular Genetics of Chronic Inflammation and Allergic Disease,Max-Delbrück Center for Molecular Medicine, ²Murdoch Childrens Research Institute, ³Department of Paediatrics,University of Melbourne, ⁴Centre for Social and Early Emotional Development, Faculty of Health,Deakin University, ⁵Department of Paediatrics,University of Western Australia

Journal (Genetics)

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Sabyasachi Dash^1,2,3, Muthukumar Balasubramaniam², Chandravanu Dash², Jui Pandhare²

¹School of Biotechnology,Kalinga Institute of Industrial Technology University, ²1. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology,Meharry Medical College, ³Kalinga Institute of Industrial Technology University

Journal (Genetics)

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Yoshiko Maida*¹, Mami Yasukawa*¹, Marco Ghilotti¹, Yoshinari Ando¹, Kenkichi Masutomi¹

¹Division of Cancer Stem Cell,National Cancer Center Research Institute

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