JOVE JOURNAL

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Showing 49 - 60 in 797 videos.

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Journal (Developmental Biology)

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

Naomi J. M. Kakiailatu¹, Laura E. Kuil^1,2, Eric Bindels³, Joke T. M. Zink³, Michael Vermeulen³, Veerle Melotte⁴, Maria M. Alves¹

¹Department of Clinical Genetics,Erasmus University Medical Center - Sophia Children's Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Journal (Developmental Biology)

Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing

Naomi J. M. Kakiailatu¹, Laura E. Kuil^1,2, Eric Bindels³, Joke T. M. Zink³, Michael Vermeulen³, Veerle Melotte⁴, Maria M. Alves¹

¹Department of Clinical Genetics,Erasmus University Medical Center - Sophia Children's Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Journal (Developmental Biology)

FACS Enrichment of Zebrafish Gut Cell Suspension for scRNAseq

Naomi J. M. Kakiailatu¹, Laura E. Kuil^1,2, Eric Bindels³, Joke T. M. Zink³, Michael Vermeulen³, Veerle Melotte⁴, Maria M. Alves¹

¹Department of Clinical Genetics,Erasmus University Medical Center - Sophia Children's Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Journal (Developmental Biology)

Dissection and Isolation of Mouse Mandibular Incisors and Associated Periodontal Tissues to Expose the Incisor Epithelial Cervical Loop

Abinaya Sundari Thooyamani*¹, Elias Shahin*², Sanako Takano¹, Amnon Sharir², Jimmy K. Hu^1,3

¹School of Dentistry,University of California Los Angeles, ²The Institute of Biomedical and Oral Research, Faculty of Dental Medicine,Hebrew University of Jerusalem, ³Molecular Biology Institute,University of California Los Angeles

Journal (Developmental Biology)

Periodontal Tissue Embedding and Timelapse Microscopy of Mouse Incisor Explant Cultures

Abinaya Sundari Thooyamani*¹, Elias Shahin*², Sanako Takano¹, Amnon Sharir², Jimmy K. Hu^1,3

¹School of Dentistry,University of California Los Angeles, ²The Institute of Biomedical and Oral Research, Faculty of Dental Medicine,Hebrew University of Jerusalem, ³Molecular Biology Institute,University of California Los Angeles

Journal (Developmental Biology)

Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal

Abinaya Sundari Thooyamani*¹, Elias Shahin*², Sanako Takano¹, Amnon Sharir², Jimmy K. Hu^1,3

¹School of Dentistry,University of California Los Angeles, ²The Institute of Biomedical and Oral Research, Faculty of Dental Medicine,Hebrew University of Jerusalem, ³Molecular Biology Institute,University of California Los Angeles

Journal (Developmental Biology)

Optogenetic Signaling Activation in Zebrafish Embryos

Allison J. Saul*¹, Catherine E. Rogers*¹, Marcial Garmendia-Cedillos², Thomas Pohida², Katherine W. Rogers¹

¹Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development,NIH, ²Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering,NIH

Journal (Developmental Biology)

Injecting bOpto-BMP/Nodal Constructs into Zebrafish Embryos

Allison J. Saul*¹, Catherine E. Rogers*¹, Marcial Garmendia-Cedillos², Thomas Pohida², Katherine W. Rogers¹

¹Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development,NIH, ²Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering,NIH

Journal (Developmental Biology)

Light Exposure Experiment to Activate BMP or Nodal Signaling in Zebrafish

Allison J. Saul*¹, Catherine E. Rogers*¹, Marcial Garmendia-Cedillos², Thomas Pohida², Katherine W. Rogers¹

¹Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development,NIH, ²Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering,NIH

Journal (Developmental Biology)

Evaluation of bOpto-BMP/Nodal Expressing Embryos After the Light Exposure Experiment

Allison J. Saul*¹, Catherine E. Rogers*¹, Marcial Garmendia-Cedillos², Thomas Pohida², Katherine W. Rogers¹

¹Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development,NIH, ²Instrumentation Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering,NIH

Journal (Developmental Biology)

Collection and Washing of Sperm from the AcroSensE Mouse Model for Real-Time Imaging

Roy Cohen^1,2, Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis^1,2

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Journal (Developmental Biology)

Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

Roy Cohen^1,2, Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis^1,2

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

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