JOVE JOURNAL

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Journal (Developmental Biology)

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Sarah Avila-Barnard, David C. Volz

¹Department of Environmental Sciences,University of California, Riverside

Journal (Developmental Biology)

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Harunobu Kagawa*¹, Alok Javali*¹, Heidar Heidari Khoei*¹, Theresa Maria Sommer³, Giovanni Sestini³, Maria Novatchkova, Yvonne Scholte op Reimer, Nicolas Rivron

¹Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA),Vienna Biocenter (VBC), ²Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), ³Vienna BioCenter PhD Program,Doctoral School of the University of Vienna and Medical University of Vienna

Journal (Developmental Biology)

Isolation of Preadipocytes from Broiler Chick Embryos

Minjeong Kim, Usuk Jung, Elizabeth Shepherd, Robert Mihelic, Brynn H. Voy

¹Department of Animal Science,University of Tennessee

Journal (Developmental Biology)

Fluorescent In Situ Hybridization and 5-Ethynyl-2′-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum

Sosuke Fujita, Erina Kuranaga, Masayuki Miura, Yu-ichiro Nakajima

¹Graduate School of Life Sciences,Tohoku University, ²Graduate School of Pharmaceutical Sciences,The University of Tokyo

Journal (Developmental Biology)

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Yang Zhang*^1,2,3, Zhe Zhang*^1,2,3, Nannan Kang^2,3, Xiaoqiang Sheng^2,3

¹Center for Reproductive Medicine and Obstetrics and Gynecology,The Affiliated Drum Tower Hospital of Nanjing University Medical School, ²State Key Laboratory of Reproductive Medicine,Nanjing Medical University, ³Center for Molecular Reproductive Medicine,Nanjing University

Journal (Developmental Biology)

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Tamara Gonzalez-Costa², Jose Luis de la Pompa², Joaquim Grego-Bessa²

¹Intercellular Signalling in Cardiovascular Development and Disease Laboratory,Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Ciber de Enfermedades Cardiovasculares

Journal (Developmental Biology)

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Miquel Sendra, Jorge Mañes, Jorge N. Domínguez³, Miguel Torres

¹Cardiovascular Regeneration Program,Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Cardiovascular Development Group, Department of Experimental Biology,University of Jaén, Jaén, ³Fundación Medina, Granada

Journal (Developmental Biology)

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Laura Adams*¹, Ying Liu*¹, Barbara Durrant, Elena Ruggeri, Carly Young, Rebecca Krisher, Irina Polejaeva

¹Department of Animal, Dairy, and Veterinary Sciences,Utah State University, ²Reproductive Sciences, Beckman Center for Conservation Research,San Diego Zoo Wildlife Alliance, ³Genus PLC

Journal (Developmental Biology)

Polysome Purification from Soybean Symbiotic Nodules

María Martha Sainz, Carla Valeria Filippi, Guillermo Eastman³, Mariana Sotelo-Silveira, C. Mauro Martinez, Omar Borsani, José Sotelo-Silveira⁴

¹Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía,Universidad de la República, ²Departamento de Genómica,Instituto de Investigaciones Biológicas Clemente Estable, ³Department of Biology,University of Virginia, ⁴Departamento de Biología Celular y Molecular, Facultad de Ciencias,Universidad de la República

Journal (Developmental Biology)

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Bridget T. Jacques-Fricke, Julaine Roffers-Agarwal³, Callie M. Gustafson³, Laura S. Gammill³

¹Department of Biology and Neuroscience Program,Hamline University, ²Department of Genetics, Cell Biology and Development,University of Minnesota, ³Developmental Biology Center,University of Minnesota

Journal (Developmental Biology)

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Yoko Tanimoto*¹, Natsuki Mikami*^1,2, Miyuki Ishida, Natsumi Iki, Kanako Kato, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno

¹Laboratory Animal Resource Center and Trans-Border Medical Research Center,University of Tsukuba, ²Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba

Journal (Developmental Biology)

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Nishant Singh*^1,2, Anubhav Prakash*¹, Suraj Ranganath Chakravarthy*¹, Raman Kaushik*¹, Raj K. Ladher

¹National Centre for Biological Sciences,Tata Institute of Fundamental Research, ²The University of Trans-Disciplinary Health Sciences and Technology

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