JOVE JOURNAL

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Journal (Developmental Biology)

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

Sergey Kurdyukov, Youhong Song, Terence W-Y Tiew, Xin-Ding Wang, Kim E. Nolan, Ray J. Rose

¹School of Environmental and Life Sciences,University of Newcastle, Callaghan, New South Wales, Australia

Journal (Developmental Biology)

Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment

Janice L. Walker, Brigid M. Bleaken, Iris M. Wolff, A. Sue Menko

¹Pathology, Anatomy and Cell Biology,Thomas Jefferson University

Journal (Developmental Biology)

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Viviana Meraviglia*¹, Alessandra Zanon*¹, Alexandros A. Lavdas, Christine Schwienbacher, Rosamaria Silipigni, Marina Di Segni, Huei-Sheng Vincent Chen, Peter P. Pramstaller, Andrew A. Hicks, Alessandra Rossini

¹Center for Biomedicine,European Academy Bozen/Bolzano (EURAC), ²Laboratory of Medical Genetics,Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, ³Del E. Webb Center for Neuroscience, Aging & Stem Cell Research,Sanford-Burnham Medical Research Institute

Journal (Developmental Biology)

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Shenglan Li*^1,2, Haipeng Xue*^1,2, Bo Long^2,5, Li Sun^2,6, Tai Truong^2,4,7, Ying Liu^2,3

¹Department of Neurosurgery,The University of Texas Health Science Center at Houston, ²Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,The University of Texas Health Science Center at Houston, ³The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine,The University of Texas Health Science Center at Houston, ⁴Summer Research Program, Office of Educational Programs,The University of Texas Health Science Center at Houston, ⁵Department of Anesthesiology,Shengjing Hospital, China Medical University, ⁶Department of Oncology, Renji Hospital,Shanghai Jiaotong University School of Medicine, ⁷Biology Department,University of West Georgia

Journal (Developmental Biology)

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick^GFPNeural Tube Grafting and Carbocyanine Dye DiI Injection

Jean-Marie Delalande², Nikhil Thapar, Alan J. Burns³

¹Birth Defects Research Centre,UCL Institute of Child Health, ²Blizard Institute, Centre for Digestive Diseases,Queen Mary University of London, Barts and The London School of Medicine and Dentistry, ³Department of Clinical Genetics,Erasmus University Medical Center, Rotterdam

Journal (Developmental Biology)

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Tae-Young Choi, Mehwish Khaliq, Sungjin Ko, Juhoon So, Donghun Shin

¹Department of Developmental Biology, McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Wikrom Wongpaiboonwattana, Marios P. Stavridis

¹Division of Cancer Research, College of Medicine, Dentistry and Nursing,University of Dundee

Journal (Developmental Biology)

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Andrea Mothe, Charles H. Tator²

¹Division of Genetics and Development,Toronto Western Research Institute and Krembil Neuroscience Center, ²Department of Surgery, Division of Neurosurgery,Toronto Western Hospital and University of Toronto

Journal (Developmental Biology)

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Daniel Prieto, Gonzalo Aparicio², Matías Machado, Flavio R. Zolessi²

¹Institut Pasteur de Montevideo, ²Sección Biología Celular, Facultad de Ciencias,Universidad de la República

Journal (Developmental Biology)

Immunostaining to Visualize Murine Enteric Nervous System Development

Amanda J. Barlow-Anacker, Christopher S. Erickson, Miles L. Epstein, Ankush Gosain^2,3

¹Department of Surgery,University of Wisconsin School of Medicine and Public Health, ²Department of Neurosciences,University of Wisconsin School of Medicine and Public Health, ³Department of Pediatrics,University of Wisconsin School of Medicine and Public Health

Journal (Developmental Biology)

Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies

Daniel Vogt, Pei-Rung Wu, Shawn F. Sorrells, Christine Arnold, Arturo Alvarez-Buylla, John L. R. Rubenstein

¹Department of Psychiatry,University of California San Francisco, ²Department of Neurological Surgery,University of California San Francisco

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