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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Evan Bardot^1,2,3, Nikos Tzavaras⁴, Deanna L. Benson^5,6, Nicole C. Dubois^1,2,3

¹Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, ²Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, ⁴Microscopy Core,Icahn School of Medicine at Mount Sinai, ⁵Department of Neuroscience,Icahn School of Medicine at Mount Sinai, ⁶Friedman Brain Institute,Icahn School of Medicine at Mount Sinai

Journal (Developmental Biology)

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

Meng Li*^1,2, Ming Hao*^1,2, Dong Jiang³, Yanxi Chen⁴, Wen Wang^1,2

¹Cellular Biomedicine Group, China, ²Cellular Biomedicine Group, California, ³Department of Orthopedics,Shanghai TCM-Integrated Hospital, ⁴Department of Orthopedics, Shanghai East Hospital,Tongji University

Journal (Developmental Biology)

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Pei-Wen Liu¹, Jia-Bao Xu¹, Yun-Qiao Dong², Xiao-Guang Chen¹, Jin-Bao Gu¹

¹Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health,Southern Medical University, ²Reproductive Medical Centre of Guangdong Women and Children's Hospital

Journal (Developmental Biology)

The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

Nan Zhang^1,2, Edward Membreno¹, Susan Raj¹, Hongjie Zhang^1,3, Liakot A Khan¹, Verena Gobel¹

¹Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children,Harvard Medical School, ²College of Life Sciences,Jilin University, ³Faculty of Health Sciences,University of Macau

Journal (Developmental Biology)

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Nan Zhang^1,2, Liakot A Khan¹, Edward Membreno¹, Gholamali Jafari¹, Siyang Yan¹, Hongjie Zhang^1,3, Verena Gobel¹

¹Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital,Harvard Medical School, ²College of Life Sciences,Jilin University, ³Faculty of Health Sciences,University of Macau

Journal (Developmental Biology)

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

Veronica Akle¹, Nathalie Agudelo-Dueñas*^1,2, Maria A. Molina-Rodriguez*¹, Laurel Brianne Kartchner^1,3,4,6, Annette Marie Ruth^1,3,5,6, John M. González³, Manu Forero-Shelton²

¹Laboratory of Neurosciences and Circadian Rhythms, School of Medicine,Universidad de los Andes, ²Biophysics Group, Department of Physics,Universidad de los Andes, ³Laboratory of Basic Medical Sciences, School of Medicine,Universidad de los Andes, ⁴Department of Microbiology and Immunology,University of North Carolina, ⁵Notre Dame Initiative for Global Development,University of Notre Dame, ⁶USAID Research and Innovation Fellowship program

Journal (Developmental Biology)

Methods for Imaging Intracellular pH of the Follicle Stem Cell Lineage in Live Drosophila Ovarian Tissue

Sumitra Tatapudy¹, Marimar Benitez¹, Todd Nystul¹

¹Departments of Anatomy and OB/GYN-RS,University of California, San Francisco

Journal (Developmental Biology)

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

Rebecca J. McFarland*¹, Sharlene P. Brown*¹, Eudorah Vital¹, Jonathan M. Werner¹, Rachel M. Brewster¹

¹Department of Biological Sciences,University of Maryland, Baltimore County

Journal (Developmental Biology)

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

Farwah Iqbal^1,2, Yarden S. Gratch¹, Peter Szaraz^1,2, Clifford L. Librach^1,2,3,4,5

¹Create Fertility Centre, ²Department of Physiology,University of Toronto, ³Department of Obstetrics and Gynecology,University of Toronto, ⁴Department of Medical Sciences,University of Toronto, ⁵Department of Obstetrics and Gynecology,Women's College Hospital

Journal (Developmental Biology)

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Prashanth Ravishankar¹, M. Alejandra Zeballos¹, Kartik Balachandran¹

¹Department of Biomedical Engineering,University of Arkansas

Journal (Developmental Biology)

Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta

Ashish Misra¹, Zhonghui Feng¹, Jiasheng Zhang¹, Zhi-Yin Lou^2,3, Daniel M. Greif¹

¹Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine,Yale University School of Medicine, ²Department of Neurology,Yale University School of Medicine, ³Department of Neurology, Xinhua Hospital,Shanghai Jiaotong University School of Medicine

Journal (Developmental Biology)

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Daisuke Kurihara*^1,2, Yusuke Kimata*¹, Tetsuya Higashiyama^1,2,3, Minako Ueda^1,3

¹Division of Biological Science, Graduate School of Science,Nagoya University, ²Higashiyama Live-Holonics Project, JST-ERATO,Nagoya University, ³Institute of Transformative Bio-Molecules (ITbM),Nagoya University

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