JOVE JOURNAL

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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Eric T. Hall, Christina A. Daly², Yan Zhang, Miriam E. Dillard, Stacey K. Ogden

¹Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, ²St. Jude Graduate School of Biomedical Sciences

Journal (Developmental Biology)

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

Michael Attwaters*¹, Jeffrey J. Kelu*¹, Tapan G. Pipalia, Simon M. Hughes

¹Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences,King’s College London

Journal (Developmental Biology)

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Andrew Berical², Mary Lou Beermann, Shingo Suzuki, Jake LeSuer, Taylor Matte, Brian Davis, Darrell Kotton², Finn Hawkins²

¹Pulmonary Center,Boston University School of Medicine, ²Center for Regenerative Medicine,Boston University and Boston Medical Center, ³Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,University of Texas Health Science Center

Journal (Developmental Biology)

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Changchun Dong*¹, Bikash Lamichhane*¹, Yongxu Zhang, Xiaofang Wang

¹Department of Biomedical Sciences,Texas A&M School of Dentistry

Journal (Developmental Biology)

FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

Giulia Riparini, James M. Simone, Vittorio Sartorelli

¹Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH), ²Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH)

Journal (Developmental Biology)

In Ovo Intravascular Injection in Chicken Embryos

Kai Jin², Jing Zhou², Gaoyuan Wu², Ziyi Lian², Zongyi Zhao², Shujian Zhou², Chen Chen², Hongyan Sun², Yingjie Niu², Qishenng Zuo², Yani Zhang², Jiuzhou Song, Guohong Chen², Bichun Li^2,4

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou University, ²Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology,Yangzhou University, ³Animal & Avian Sciences,University of Maryland, College Park, ⁴College of Biotechnology,Jiangsu University of Science and Technology

Journal (Developmental Biology)

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

Connor D. Johnson, Lylybell Y. Zhou, Daniel Kopinke²

¹Department of Pharmacology and Therapeutics,University of Florida College of Medicine, ²Myology Institute,University of Florida College of Medicine

Journal (Developmental Biology)

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Hemin P. Shah, Olivier Devergne

¹Department of Biological Sciences,Northern Illinois University

Journal (Developmental Biology)

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Wei Zhang*¹, Xinjuan Lei*¹, Xin Zhou*^2,3, Boling He, Liqin Xiao, Huimin Yue, Shulin Wang, Yuting Sun, Yajun Wu, Liyang Wang⁴, George Ghartey-Kwansah, Odell D. Jones, Joseph L. Bryant, MengMeng Xu, Jianjie Ma, Xuehon Xu

¹National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB,Shaanxi Normal University College of Life Sciences, ²Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine,Xi'an Medical University, ³Ohio State University College of Medicine, ⁴Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School, ⁵University of Pennsylvania ULAR, ⁶University of Maryland School of Medicine, ⁷Columbia University Medical Center

Journal (Developmental Biology)

Investigating Scarless Tissue Regeneration in Embryonic Wounded Chick Corneas

Manish Pathuri, James Spurlin III, Peter Lwigale, Tyler Schwend

¹Department of Biology,Illinois Wesleyan University, ²Department of Biosciences,Rice University

Journal (Developmental Biology)

Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

Rhiannon B. Werder*^1,2,3, Jessie Huang*^1,2, Kristine M. Abo², Olivia T. Hix², Kasey Minakin², Konstantinos-Dionysios Alysandratos², Carly Merritt², Kayleigh Berthiaume², Andrea B. Alber², Claire L. Burgess², Darrell N. Kotton*^1,2, Andrew A. Wilson*^1,2

¹Center for Regenerative Medicine,Boston University and Boston Medical Center, ²The Pulmonary Center and Department of Medicine,Boston University School of Medicine, ³QIMR Berghofer Medical Research Institute

Journal (Developmental Biology)

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Lara Hemeryck, Ivo Lambrichts, Annelies Bronckaers, Hugo Vankelecom

¹Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute,KU Leuven (University of Leuven), ²Biomedical Research Institute (BIOMED),UHasselt, Hasselt University

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