JOVE JOURNAL

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Journal (Cancer Research)

Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor

Brittany Turner-Ivey¹, Jody Fromm Longo², Dorea P. Jenkins², Stephen T. Guest³, Steven L. Carroll^1,2

¹Hollings Cancer Center,Medical University of South Carolina, ²Department of Pathology and Laboratory Medicine,Medical University of South Carolina, ³Department of Computational Medicine and Bioinformatics,University of Michigan, Ann Arbor

Journal (Cancer Research)

Hepatocellular Carcinoma Tissue Dissociation for Organoid Culture

Cheng-Yang Zhang*^1,2, Xiao-Feng Zhang*³, Jun Yuan*^1,2, Yuan-Feng Gong⁴, Hui Tang⁴, Wan-Yu Guo^1,2, Tong-Yan Li^1,2, Cai-Wen Li^1,2, Yun-Qiang Tang⁴, Ning-Fang Ma^1,2, Ming Liu^1,2

¹Affiliated Cancer Hospital and Institute of Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Center for Cancer Research and Translational Medicine, School of Basic Medical Sciences,Guangzhou Medical University, ³Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center,Guangzhou Medical University, ⁴Department of Hepatobiliary Surgery, Affiliated Cancer Hospital,Guangzhou Medical University

Journal (Cancer Research)

Hepatocellular Carcinoma Organoid Culture and Digestion to Study Liver Cancer Pathogenesis

Cheng-Yang Zhang*^1,2, Xiao-Feng Zhang*³, Jun Yuan*^1,2, Yuan-Feng Gong⁴, Hui Tang⁴, Wan-Yu Guo^1,2, Tong-Yan Li^1,2, Cai-Wen Li^1,2, Yun-Qiang Tang⁴, Ning-Fang Ma^1,2, Ming Liu^1,2

¹Affiliated Cancer Hospital and Institute of Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Center for Cancer Research and Translational Medicine, School of Basic Medical Sciences,Guangzhou Medical University, ³Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center,Guangzhou Medical University, ⁴Department of Hepatobiliary Surgery, Affiliated Cancer Hospital,Guangzhou Medical University

Journal (Cancer Research)

Development and Optimization of A Human Hepatocellular Carcinoma Patient-Derived Organoid Model for Potential Target Identification and Drug Discovery

Cheng-Yang Zhang*^1,2, Xiao-Feng Zhang*³, Jun Yuan*^1,2, Yuan-Feng Gong⁴, Hui Tang⁴, Wan-Yu Guo^1,2, Tong-Yan Li^1,2, Cai-Wen Li^1,2, Yun-Qiang Tang⁴, Ning-Fang Ma^1,2, Ming Liu^1,2

¹Affiliated Cancer Hospital and Institute of Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Center for Cancer Research and Translational Medicine, School of Basic Medical Sciences,Guangzhou Medical University, ³Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center,Guangzhou Medical University, ⁴Department of Hepatobiliary Surgery, Affiliated Cancer Hospital,Guangzhou Medical University

Journal (Cancer Research)

High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

Eliza Guti*^1,2, Ákos Máté Bede*^1,3, Csongor Váróczy*^1,3, Csaba Hegedűs¹, Máté Á. Demény^1,4, László Virág^1,4

¹Department of Medical Chemistry, Faculty of Medicine,University of Debrecen, ²Doctoral School of Molecular Medicine,University of Debrecen, ³National Academy of Scientist Education,University of Debrecen, ⁴ELKH-DE Cell Biology and Signaling Research Group

Journal (Cancer Research)

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Mark A. J. Gorris^1,2, Evgenia Martynova*^1,3, Mark W. D. Sweep*^1,4, Iris A. E. van der Hoorn^1,5, Shabaz Sultan^1,3, Mike J. D. E. Claassens¹, Lieke L. van der Woude^1,2,6, Kiek Verrijp^1,2,6, Carl G. Figdor^1,3, Johannes Textor^1,3, I. Jolanda M. de Vries¹

¹Department of Medical BioSciences,Radboudumc, ²Division of Immunotherapy, Oncode Institute,Radboudumc, ³Data Science, Institute for Computing and Information Sciences,Radboud University, ⁴Department of Medical Oncology,Radboudumc, ⁵Department of Pulmonary Diseases,Radboudumc, ⁶Department of Pathology,Radboudumc

Journal (Cancer Research)

Workflow for Immune Cells Detection Using the Machine Learning Algorithm ImmuNet

Mark A. J. Gorris^1,2, Evgenia Martynova*^1,3, Mark W. D. Sweep*^1,4, Iris A. E. van der Hoorn^1,5, Shabaz Sultan^1,3, Mike J. D. E. Claassens¹, Lieke L. van der Woude^1,2,6, Kiek Verrijp^1,2,6, Carl G. Figdor^1,3, Johannes Textor^1,3, I. Jolanda M. de Vries¹

¹Department of Medical BioSciences,Radboudumc, ²Division of Immunotherapy, Oncode Institute,Radboudumc, ³Data Science, Institute for Computing and Information Sciences,Radboud University, ⁴Department of Medical Oncology,Radboudumc, ⁵Department of Pulmonary Diseases,Radboudumc, ⁶Department of Pathology,Radboudumc

Journal (Cancer Research)

Systematic Endobronchial Ultrasound – The Six Landmarks Approach

Anne Orholm Nielsen¹, Kristoffer Mazanti Cold¹, Anishan Vamadevan¹, Lars Konge¹, Paul Frost Clementsen¹

¹Copenhagen Academy for Medical Education and Simulation (CAMES),Rigshospitalet, University of Copenhagen

Journal (Cancer Research)

Endobronchial Ultrasound-Guided Sampling of Lymph Nodes Using Transbronchial Needle Aspiration

Anne Orholm Nielsen¹, Kristoffer Mazanti Cold¹, Anishan Vamadevan¹, Lars Konge¹, Paul Frost Clementsen¹

¹Copenhagen Academy for Medical Education and Simulation (CAMES),Rigshospitalet, University of Copenhagen

Journal (Cancer Research)

Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion

Madeline Friedman-DeLuca^1,2, Prachiben P. Patel^1,2, Burcu Karadal-Ferrena^1,2, Nicole D. Barth^1,2, Camille L. Duran^1,2, Xianjun Ye^1,2,3, Michael Papanicolaou^2,4, John S. Condeelis^2,3,4,5,6,7, Maja H. Oktay^1,2,3,5,6,7, Lucia Borriello*^1,2,8, David Entenberg*^1,2,3,5,6

¹Department of Pathology,Albert Einstein College of Medicine/Montefiore Medical Center, ²Cancer Dormancy and Tumor Microenvironment Institute,Albert Einstein College of Medicine/Montefiore Medical Center, ³Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine/Montefiore Medical Center, ⁴Department of Cell Biology,Albert Einstein College of Medicine/Montefiore Medical Center, ⁵Montefiore Einstein Cancer Center,Albert Einstein College of Medicine/Montefiore Medical Center, ⁶Integrated Imaging Program for Cancer Research,Albert Einstein College of Medicine/Montefiore Medical Center, ⁷Department of Surgery,Albert Einstein College of Medicine/Montefiore Medical Center, ⁸Department of Cancer and Cellular Biology, Lewis Katz School of Medicine,Fox Chase Cancer Center

Journal (Cancer Research)

Surgical Protocol for the Selective Photoconversion of Lung Metastases

Madeline Friedman-DeLuca^1,2, Prachiben P. Patel^1,2, Burcu Karadal-Ferrena^1,2, Nicole D. Barth^1,2, Camille L. Duran^1,2, Xianjun Ye^1,2,3, Michael Papanicolaou^2,4, John S. Condeelis^2,3,4,5,6,7, Maja H. Oktay^1,2,3,5,6,7, Lucia Borriello*^1,2,8, David Entenberg*^1,2,3,5,6

¹Department of Pathology,Albert Einstein College of Medicine/Montefiore Medical Center, ²Cancer Dormancy and Tumor Microenvironment Institute,Albert Einstein College of Medicine/Montefiore Medical Center, ³Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine/Montefiore Medical Center, ⁴Department of Cell Biology,Albert Einstein College of Medicine/Montefiore Medical Center, ⁵Montefiore Einstein Cancer Center,Albert Einstein College of Medicine/Montefiore Medical Center, ⁶Integrated Imaging Program for Cancer Research,Albert Einstein College of Medicine/Montefiore Medical Center, ⁷Department of Surgery,Albert Einstein College of Medicine/Montefiore Medical Center, ⁸Department of Cancer and Cellular Biology, Lewis Katz School of Medicine,Fox Chase Cancer Center

Journal (Cancer Research)

Magnetic Fluorescent Bead-Based Dual-Reporter Flow Analysis of PDL1-Vaxx Peptide Vaccine-Induced Antibody Blockade of the PD-1/PD-L1 Interaction

Jay Overholser*¹, Linlin Guo*¹, Pravin T. P. Kaumaya^1,2

¹Department of Obstetrics & Gynecology,The Ohio State University Wexner Medical Center, ²The James Comprehensive Cancer Center

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