JOVE JOURNAL

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Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Meng-Fei Xu^1,2, Yu-Hao Yang^1,2, Ya-Lan Wei^3,4, Jing-Lian Zhang^1,2, Xuan Lin^1,2, Xiao-Yan Lin^1,2, Hui Chen^1,2, Zhen-Yu She^1,2

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³Fujian Obstetrics and Gynecology Hospital, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital,Affiliated Hospital of Fujian Medical University

Journal (Biology)

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Neelima Boora*¹, Vibha Verma*¹, Ridhi Khurana¹, Gautam Gawande¹, Sanchi Bhimrajka¹, Komal Chaprana¹, Meenu Kapoor², Sanjay Kapoor¹

¹Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology,University of Delhi South Campus, ²University School of Biotechnology,Guru Gobind Singh Indraprastha University

Journal (Biology)

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Yoshika Janapala*¹, Katrina Woodward*¹, Jiwon Lee², Melanie Rug², Thomas Preiss^1,3, Nikolay E. Shirokikh¹

¹Division of Genome Sciences and Cancer, The John Curtin School of Medical Research,The Australian National University, ²Centre for Advanced Microscopy,The Australian National University, ³Victor Chang Cardiac Research Institute

Journal (Biology)

Versatility of Protocols for Resistance Training and Assessment Using Static and Dynamic Ladders in Animal Models

Eduardo Iglesias-Gutiérrez^1,2, Manuel Fernández-Sanjurjo², Álvaro F. Fernández³, Francisco José Rodríguez Díaz⁴, Isabel López-Taboada^5,6, Cristina Tomás-Zapico^1,2, Benjamín Fernández-García^2,7

¹Department of Functional Biology,University of Oviedo, ²Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ³Department of Biochemistry and Molecular Biology,University of Oviedo, ⁴Animal facilities,University of Oviedo, ⁵Department of Psychology,University of Oviedo, ⁶Instituto de Neurociencias del Principado de Asturias (INEUROPA),University of Oviedo, ⁷Department of Morphology and Cellular Biology,University of Oviedo

Journal (Biology)

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Qianqian Guo¹, Kunimaro Furuta¹, Ahmed Aly¹, Samar H. Ibrahim^1,2

¹Division of Gastroenterology & Hepatology,Mayo Clinic, ²Division of Pediatric Gastroenterology,Mayo Clinic

Journal (Biology)

Spinal Cord Transection In Xenopus laevis Tadpoles

Paula G. Slater¹, Juan Larraín¹

¹Center for Aging and Regeneration, Facultad de Ciencias Biológicas,P. Universidad Católica de Chile

Journal (Biology)

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Huifei Sophia Zheng¹, Chen-Che Jeff Huang¹

¹Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine,Auburn University

Journal (Biology)

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Sebastian Bass-Stringer^1,2, Colleen J. Thomas^2,3, Clive N. May³, Paul Gregorevic⁴, Kate L. Weeks^1,5,6, Julie R. McMullen^1,2,5,6,7

¹Baker Heart and Diabetes Institute, ²Department of Physiology, Anatomy and Microbiology,La Trobe University, ³Florey Institute of Neuroscience and Mental Health,University of Melbourne, ⁴Department of Physiology, Centre for Muscle Research (CMR),The University of Melbourne, ⁵Department of Diabetes, Central Clinical School,Monash University, ⁶Baker Department of Cardiometabolic Health,The University of Melbourne, ⁷Department of Physiology and Department of Medicine Alfred Hospital,Monash University

Journal (Biology)

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Robert C. Moseley¹, Sophia Campione¹, Bree Cummins², Francis Motta³, Steven B. Haase¹

¹Department of Biology,Duke University, ²Department of Mathematical Sciences,Montana State University, ³Department of Mathematical Sciences,Florida Atlantic University

Journal (Biology)

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Amnah Alzahmi^1,2, Sarah Daakour¹, Diana Charles El Assal³, Bushra S. Dohai³, Amphun Chaiboonchoe³, Weiqi Fu^3,4, David R. Nelson¹, Alexandra Mystikou¹, Kourosh Salehi-Ashtiani^1,3

¹Center for Genomics and Systems Biology (CGSB),New York University Abu Dhabi Research Institute, ²Department of Biology,United Arab Emirates University, ³Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math,New York University Abu Dhabi, ⁴Department of Marine Science, Ocean College,Zhejiang University

Journal (Biology)

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Eleonora Rizzi*¹, Clémence Deligne*¹, Lucie Dehouck¹, Roberta Bilardo², Yasutero Sano³, Fumitaka Shimizu³, Takashi Kanda³, Marina Resmini², Fabien Gosselet¹, Marie-Pierre Dehouck¹, Caroline Mysiorek¹

¹Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), ²Queen Mary University of London, Department of Chemistry, ³Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience

Journal (Biology)

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Ayushi Verma*¹, Vipin Kumar*¹, Saicharan Ghantasala¹, Shuvolina Mukherjee¹, Sanjeeva Srivastava¹

¹Department of Biosciences and Bioengineering,Indian Institute of Technology Bombay

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