JOVE JOURNAL

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Two-photon Imaging of Intracellular Ca²⁺ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta

Bradley T. Endres^1,2, Alexander Staruschenko¹, Marie Schulte⁴, Aron M. Geurts^1,2,3, Oleg Palygin¹

¹Departments of Physiology,Medical College of Wisconsin, ²Human and Molecular Genetics Center,Medical College of Wisconsin, ³Cardiovascular Center,Medical College of Wisconsin, ⁴Blood Research Institute of Wisconsin

Journal (Biology)

SpOT the Correct Tissue Every Time in Multi-tissue Blocks

Anna Coffey^1,2, Michael D. Johnson³, Deborah L. Berry³

¹Center for Advanced Preclinical Research (CAPR),Frederick National Laboratory for Cancer Research, ²Leidos Biomedical Research, Inc., ³Department of Oncology,Georgetown University

Journal (Biology)

qPCRTag Analysis – A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Leslie A. Mitchell¹, Nick A. Phillips¹, Andrea Lafont², James A. Martin¹, Rupal Cutting², Jef D. Boeke¹

¹Department of Biochemistry and Molecular Pharmacology,Institute for Systems Genetics, ²Roche Life Science, USA

Journal (Biology)

Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay

Justin M. Wolter^1,2, Kasuen Kotagama^1,2, Cody S. Babb², Marco Mangone^1,2

¹School of Life Sciences,Arizona State University, ²Biodesign Institute,Arizona State University

Journal (Biology)

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Lei Fang^1,2, Albert Neutzner^1,2, Stephanie Turtschi¹, Josef Flammer¹, Maneli Mozaffarieh¹

¹Department of Ophthalmology,University of Basel, ²Department of Biomedicine,University of Basel

Journal (Biology)

Sampling Blood from the Lateral Tail Vein of the Rat

Graham Lee¹, Ki A. Goosens^1,2

¹McGovern Institute for Brain Research,Massachusetts Institute of Technology, ²Department of Brain and Cognitive Sciences,Massachusetts Institute of Technology

Journal (Biology)

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Percy Griffin^1,2, Ashley Sexton³, Lauren Macneill³, Yoshie Iizuka^3,4, Michael K. Lee^1,2, Martina Bazzaro^3,4

¹Department of Neuroscience,University of Minnesota, ²Institute for Translational Neuroscience,University of Minnesota, ³Department of Obstetrics, Gynecology, and Women’s Heath,University of Minnesota, ⁴Masonic Cancer Center,University of Minnesota

Journal (Biology)

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates

Elite Possik^1,2, Arnim Pause^1,2

¹Goodman Cancer Research Center,McGill University, ²Department of Biochemistry,McGill University

Journal (Biology)

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique

Guillaume Luxardi¹, Brian Reid¹, Fernando Ferreira^1,2, Pauline Maillard³, Min Zhao^1,4

¹Department of Dermatology, Institute for Regenerative Cures,University of California, Davis, ²Departamento de Biologia, Centro de Biologia Molecular e Ambiental,Universidade do Minho, ³Department of Neurology and Center for Neuroscience,University of California, Davis Imaging of Dementia and Aging Laboratory, ⁴Department of Ophthalmology, Institute for Regenerative Cures,University of California, Davis

Journal (Biology)

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Kentaro Ohkuni¹, Yoshimitsu Takahashi¹, Munira A. Basrai¹

¹Genetics Branch, Center for Cancer Research,National Cancer Institute, National Institute of Health

Journal (Biology)

A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy

Victoria C Cogger*^1,2,3, Jennifer N O'Reilly*^1,2, Alessandra Warren^1,2,3, David G Le Couteur^1,2,3

¹Centre for Education and Research on Ageing & ANZAC Research Institute,University of Sydney and Concord Hospital, ²Ageing and Alzheimers Institute,Concord Hospital, ³Charles Perkins Centre,University of Sydney

Journal (Biology)

Measurement of Larval Activity in the Drosophila Activity Monitor

Aidan L. McParland¹, Taylor L. Follansbee¹, Geoffrey K. Ganter¹

¹Department of Biology,University of New England

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