JOVE JOURNAL

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Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Wells B. LaRiviere^1,2, Xiaorui Han^3,4, Kaori Oshima¹, Sarah A. McMurtry¹, Robert J. Linhardt³, Eric P. Schmidt^1,5

¹Department of Medicine,University of Colorado Anschutz Medical Campus, ²Medical Scientist Training Program,University of Colorado Anschutz Medical Campus, ³Department of Chemistry and Chemical Biology,Rensselaer Polytechnic Institute, ⁴Department of Health Sciences,Curtin University, ⁵Department of Medicine,Denver Health Medical Center

Journal (Biology)

Construction of a Realistic, Whole-Body, Three-Dimensional Equine Skeletal Model using Computed Tomography Data

Alexander K. K. Lee¹, Elizabeth W. Uhl², Michelle L. Osborn¹

¹Department of Comparative Biomedical Sciences,Louisiana State University School of Veterinary Medicine, ²Department of Pathology,University of Georgia College of Veterinary Medicine

Journal (Biology)

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

William C. Budell¹, Luis Allegri², Venkat Dandey¹, Clinton S. Potter¹, Bridget Carragher¹

¹The National Resource for Automated Molecular Microscopy,Simons Electron Microscopy Center, New York Structural Biology Center, ²piTree Software

Journal (Biology)

Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities

Morgane Dos Santos¹, Vincent Paget², François Trompier³, Gaëtan Gruel¹, Fabien Milliat²

¹Department of RAdiobiology and Regenerative MEDicine (SERAMED), Laboratory of Radiobiology of Accidental Exposures (LRAcc),Institute for Radiological Protection and Nuclear Safety (IRSN), ²Department of RAdiobiology and Regenerative MEDicine (SERAMED), Laboratory of MEDical Radiobialogy (LRMed),Institute for Radiological Protection and Nuclear Safety (IRSN), ³Department of DOSimetry (SDOS), Ionizing Radiation Dosimetry Laboratory (LDRI),Institute for Radiological Protection and Nuclear Safety (IRSN)

Journal (Biology)

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Sören S. Hüttner¹, Christian Hayn¹, Hellen E. Ahrens¹, Manuel Schmidt¹, Henriette Henze¹, Julia von Maltzahn¹

¹Leibniz Institute on Aging, Fritz-Lipmann-Institute

Journal (Biology)

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Blanca Chinchilla¹, Heran Getachew¹, Rosario Fernandez-Godino¹

¹Ocular Genomics Institute of Massachusetts Eye and Ear,Harvard Medical School

Journal (Biology)

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay

Amelia N. Gray¹, Breeana M. Ramirez¹, Selom K. Mawugbe¹, Jordan F. Mar¹, Yun-Lan C. Wong¹, Kevin S. Huang¹

¹Department of Biology and Chemistry,Azusa Pacific University

Journal (Biology)

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Chithra K Muraleedharan¹, Jay Mierzwiak¹, Darius Feier¹, Asma Nusrat¹, Miguel Quiros¹

¹Department of Pathology, School of Medicine,University of Michigan

Journal (Biology)

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

Louisiane Perrin¹, Theodore Tucker¹, Bojana Gligorijevic^1,2

¹Bioengineering Department,Temple University, ²Cancer Biology Program,Fox Chase Cancer Center

Journal (Biology)

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

Ernest O. N. Phillips*¹, Serena Giovinazzi*¹, Sarah L. Menz*¹, Yuna Son¹, Akash Gunjan¹

¹Department of Biomedical Sciences,Florida State University College of Medicine

Journal (Biology)

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Andrea Galmozzi^1,2, Bernard P. Kok¹, Enrique Saez¹

¹Department of Molecular Medicine,The Scripps Research Institute, La Jolla, CA, USA, ²Department of Medicine,University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

Journal (Biology)

A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications

Jiajing Qiu*^1,2, Yinhui Ren*^1,2, Lingxiao Zhao^1,2, Biao Zhang^1,2, Cunxu Wei^1,2

¹Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Yangzhou University, ²Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education,Yangzhou University

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