JOVE JOURNAL

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Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring

Ashutosh Dhingra*¹, Joachim Täger*¹, Elisangela Bressan*¹, Salvador Rodriguez-Nieto¹, Manmeet-Sakshi Bedi¹, Stefanie Bröer¹, Eldem Sadikoglou¹, Noémia Fernandes², Melissa Castillo-Lizardo¹, Patrizia Rizzu², Peter Heutink^1,3

¹Genome Biology of Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), ²Applied Genomics for Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), ³Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research,University of Tübingen

Journal (Biology)

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Lim Chee Liew^1,2, Yan Wang^1,2, Marta Peirats-Llobet¹, Oliver Berkowitz^1,2,3, James Whelan^1,2,3, Mathew G. Lewsey^1,3

¹Department of Animal, Plant and Soil Science, AgriBio Building,La Trobe University, ²Australian Research Council Centre of Excellence in Plant Energy Biology,La Trobe University, ³Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building,La Trobe University

Journal (Biology)

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Deepika Mittal¹, Shruti Mishra¹, Ramgopal Prajapati¹, Jyothilakshmi Vadassery¹

¹National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India

Journal (Biology)

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Giulia Bertolin¹, Gilles Le Marchand¹, Marc Tramier¹

¹Univ Rennes, CNRS, IGDR (Genetics and Development Institute of Rennes), UMR 6290, F-35000 Rennes, France

Journal (Biology)

Planarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing

Ziad Sabry¹, Christina Rabeler¹, Danielle Ireland¹, Kevin Bayingana¹, Eva-Maria S. Collins^1,2

¹Biology Department,Swarthmore College, ²Physics Department,UC San Diego

Journal (Biology)

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Sangjin Kim^1,2,3,4, Kavya Vaidya^1,2

¹Department of Physics,University of Illinois at Urbana-Champaign, ²Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, ³Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, ⁴Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign

Journal (Biology)

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Gabriela Gorczyca¹, Kamil Wartalski², Zbigniew Tabarowski³, Malgorzata Duda¹

¹Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow, ²Department of Histology,Jagiellonian University Medical College, ³Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology,Jagiellonian University in Krakow

Journal (Biology)

Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging

Hae Jin Kang¹, Hye Young Kim¹

¹Center for Vascular Research,Institute for Basic Science

Journal (Biology)

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

Bindu Konda¹, Apoorva Mulay¹, Changfu Yao¹, Stephen Beil¹, Edo Israely¹, Barry R. Stripp¹

¹Lung and Regenerative Medicine Institutes, Department of Medicine,Cedars-Sinai Medical Center

Journal (Biology)

Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

Evan S. Hill*^1,2, Jeffrey W. Brown*^1,2, William N. Frost^1,2

¹Cell Biology and Anatomy, Chicago Medical School,Rosalind Franklin University of Medicine and Science, ²Center for Brain Function and Repair,Rosalind Franklin University of Medicine and Science

Journal (Biology)

A 3D Printed Pollen Trap for Bumble Bee (Bombus) Hive Entrances

Houston J. Judd^1,2, Craig Huntzinger², Ricardo Ramirez¹, James P. Strange^2,3

¹Department of Biology,Utah State University, ²USDA-ARS Pollinating Insect Research Unit, ³Department of Entomology,The Ohio State University

Journal (Biology)

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Lourdes Mateos-Hernández¹, Sabine Rakotobe¹, Baptiste Defaye^1,2,3, Alejandro Cabezas-Cruz¹, Ladislav Šimo¹

¹UMR BIPAR, INRAE, Ecole Nationale Vétérinaire d'Alfort, ANSES,Université Paris-Est, ²Faculté de Pharmacie,Université de Limoges, ³UMR SPE 6134 CNRS,Université de Corte Pascal Paoli

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