JOVE JOURNAL

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Image Rendering Techniques in Postmortem Computed Tomography: Evaluation of Biological Health and Profile in Stranded Cetaceans

Brian C. W. Kot^1,2, Derek K. P. Chan³, Tabris Y. T. Chung¹, Henry C. L. Tsui¹

¹State Key Laboratory of Marine Pollution,City University of Hong Kong, ²Department of Veterinary Clinical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences,City University of Hong Kong, ³School of Medical and Health Sciences,Tung Wah College

Journal (Biology)

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Anthony L. Hessel^1,4, Daniel Hahn^1,2, Markus de Marées³

¹Faculty of Sport Science, Human Movement Science,Ruhr University Bochum, ²School of Human Movement and Nutrition Sciences,The University of Queensland, ³Faculty of Sport Science, Sports Medicine and Sports Nutrition,Ruhr University Bochum, ⁴Institute of Physiology II,University of Muenster

Journal (Biology)

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Yangyang Deng*^1,2, Ming Zhou*^1,3, Ying Lu¹, Jiao Chen¹, Zuhui Pu⁴, Dongjing Yu^1,5, Yifan Dai⁶, Yongqiang Zhan⁷, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center,Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, ²Xianning Hospital of Traditional Chinese Medicine, ³Liver-biotechnology (Shenzhen) Co., Ltd., ⁴Department of Radiology,Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, ⁵Department of Life Sciences,University of Toronto, ⁶Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University, ⁷Department of Hepatopancreatobiliary Surgery,Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital

Journal (Biology)

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Patricia Blach^1,2, Sercan Keskin¹, Niels de Jonge^1,2

¹INM-Leibniz Institute for New Materials, ²Department of Physics,Saarland University

Journal (Biology)

Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Hugo Lachuer^1,2,3, Pallavi Mathur^1,2,3, Kevin Bleakley⁴, Kristine Schauer^1,2,3

¹Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group,Institut Curie, 75005 Paris, France, ²PSL Research University, Paris, France, ³Sorbonne Université, Paris, France, ⁴INRIA, Université Paris-Sud, PSL

Journal (Biology)

A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

Xinyi Liu^1,2, Si Wu^1,2, Wenqian Li^1,2, Meihong Zhang^1,2, Yan Wu^1,2, Ning Zhou³, Pa Wu^1,2

¹Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science,Hunan Normal University, ²State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science,Hunan Normal University, ³Department of Infectious Diseases, the Second Xiangya Hospital,Central South University

Journal (Biology)

Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans

Margarita Elena Papandreou*^1,2, Konstantinos Palikaras*^1,2, Nektarios Tavernarakis^1,2

¹Institute of Molecular Biology and Biotechnology,Foundation for Research and Technology-Hellas, Greece, ²Department of Basic Sciences, Faculty of Medicine,University of Crete, Heraklion, 70013, Crete, Greece

Journal (Biology)

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Wojciech Wiertelak¹, Mariusz Olczak¹, Dorota Maszczak-Seneczko¹

¹Laboratory of Biochemistry, Faculty of Biotechnology,University of Wroclaw

Journal (Biology)

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Megan E. Meuti¹, Robert Harrell²

¹Department of Entomology,The Ohio State University, ²Insect Transformation Facility, Institute for Bioscience and Biotechnology Research,University of Maryland

Journal (Biology)

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Julia Ratke*¹, Franziska Krämer*¹, Frederic Strobl¹

¹Physical Biology/Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt, Macromolecular Complexes (CEF - MC),Goethe-Universität

Journal (Biology)

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Mario Buenrostro-Jauregui*¹, Alejandro Tapia-de-Jesús¹, Jesus Mata¹, Luis Miguel Rodríguez-Serrano¹, María Elena Chávez-Hernández¹, Florencia Mata¹, Marco Monroy-Plasencia^1,2, Aixchell Camila Alonso-Flores¹, Perla Leal-Galicia*¹

¹Laboratorio de Neurociencias, Departamento de Psicología,Universidad Iberoamericana Ciudad de México, ²Facultad de Ciencias,Universidad Autónoma del Estado de México

Journal (Biology)

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Rupam Ghosh*¹, Jaka Kragelj*¹, Yiling Xiao*¹, Kendra K. Frederick^1,2

¹Department of Biophysics,University of Texas Southwestern Medical Center, ²Center for Alzheimer's and Neurodegenerative Disease and Center for Systems Biology,University of Texas Southwestern Medical Center

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