JOVE JOURNAL

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A High Output Method to Isolate Cerebral Pericytes from Mouse

Anupriya Mehra¹, Lucie Dehouck¹, Elodie Vandenhaute¹, Marc Fatar², Laurence Fenart¹, Fabien Gosselet¹

¹Laboratory of the Blood Brain Barrier,University of Artois, ²Department of Neurology, Universitätsmedizin Mannheim,Heidelberg University

Journal (Biology)

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Chuanxian Wei*¹, Yan Yan*¹, Xiaoli Miao¹, Renjie Jiao^1,2,3

¹Sino-French Hoffmann Institute, School of Basic Medical Sciences,Guangzhou Medical University, ²The Second Affiliated Hospital of Guangzhou Medical University, ³The State Key Laboratory of Respiratory Disease,Guangzhou Medical University

Journal (Biology)

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Mukul Girotra*^1,2, Anne-Christine Thierry³, Alexandre Harari^1,3, George Coukos¹, Olaia Naveiras^2,4, Nicola Vannini*¹

¹Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne,University of Lausanne, ²Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences,Swiss Federal Institute of Technology Lausanne (EPFL), ³Center of Experimental Therapeutics, Department of Oncology,Centre Hospitalier Universitaire Vaudois, ⁴Hematology Service, Department of Oncology,Centre Hospitalier Universitaire Vaudois (CHUV)

Journal (Biology)

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Katerina Korelova¹, Marketa Jirouskova¹, Lenka Sarnova¹, Martin Gregor¹

¹Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

Journal (Biology)

Immunolabelling Myofiber Degeneration in Muscle Biopsies

Maximilien Bencze^1,2, Baptiste Periou¹, Yasmine Baba-Amer¹, Francois J Authier¹

¹Inserm,Institut Mondor de Recherche Biomédicale, ²The Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Program,UCL Great Ormond Street Institute of Child Health

Journal (Biology)

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi

Alexander Lichius¹, Susanne Zeilinger¹

¹Department of Microbiology,University of Innsbruck

Journal (Biology)

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Ri Youn Kim*^1,2, Ju Hee Lee*^3,4, Yena Oh^1,2, Hoon-Ki Sung^3,4,5, Kyoung-Han Kim^1,2

¹University of Ottawa Heart Institute, ²Department of Cellular and Molecular Medicine,University of Ottawa, ³Translational Medicine Program,The Hospital for Sick Children, ⁴Department of Laboratory Medicine and Pathobiology,University of Toronto, ⁵Banting and Best Diabetes Centre,University of Toronto

Journal (Biology)

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Alexander Hamilton^1,2, Elisa Vergari¹, Caroline Miranda³, Andrei I. Tarasov^1,4,5

¹Oxford Centre for Diabetes, Endocrinology and Metabolism,University of Oxford, ²Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre,Malmö University HospitalMalmö, ³Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit,University of Göteborg, ⁴Oxford National Institute for Health Research,Biomedical Research Centre, ⁵Life and Medical Sciences,University of Hertfordshire

Journal (Biology)

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Naira Movsisyan^1,2, Luis A. Pardo¹

¹AG Oncophysiology,Max-Planck Institute of Experimental Medicine, ²Göttingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB)

Journal (Biology)

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

Moreno Zamai¹, Antonio Trullo², Elvira Arza¹, Ugo Cavallaro³, Valeria R. Caiolfa^1,2

¹Unit of Microscopy and Dynamic Imaging,Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain, ²Centro di Imaging Sperimentale,Ospedale San Raffaele, Milan, Italy, ³Unit of Gynecological Oncology Research,European Institute of Oncology IRCCS, Milan, Italy

Journal (Biology)

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Christian S. Thudium*¹, Amalie Engstrom*^1,2, Solveig S. Groen¹, Morten A. Karsdal¹, Anne-Christine Bay-Jensen¹

¹Nordic Bioscience, ²Department of Biomedical Sciences,University of Copenhagen

Journal (Biology)

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Grant A. R. Gale*^1,2,3, Alejandra A. Schiavon Osorio*^1,2, Anton Puzorjov*^1,2, Baojun Wang^2,3, Alistair J. McCormick^1,2

¹Institute of Molecular Plant Sciences, School of Biological Sciences,University of Edinburgh, ²Centre for Synthetic and Systems Biology,University of Edinburgh, ³Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences,University of Edinburgh

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