JOVE JOURNAL

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Showing 1,849 - 1,860 in 3,287 videos.

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Journal (Biology)

Measuring the Osmotic Water Permeability Coefficient (P_f) of Spherical Cells: Isolated Plant Protoplasts as an Example

Arava Shatil-Cohen¹, Hadas Sibony¹, Xavier Draye², François Chaumont³, Nava Moran¹, Menachem Moshelion¹

¹The RH Smith Institute of Plant Sciences and Genetics in Agriculture,The Hebrew University of Jerusalem, ²Earth and Life Institute,Université catholique de Louvain, ³Institut des Sciences de la Vie,Université catholique de Louvain

Journal (Biology)

A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening

Jeroen Aerts*¹, Julie Nys*¹, Lutgarde Arckens¹

¹Department of Biology,KU Leuven - University of Leuven

Journal (Biology)

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Sandy Chevrier¹, Romain Boidot¹

¹Department of Biology and Pathology of Tumors, Unit of Molecular Biology, Platform of Immunomonitoring and Genetics,Centre Georges-François Leclerc

Journal (Biology)

In vitro Methylation Assay to Study Protein Arginine Methylation

Rama Kamesh Bikkavilli¹, Sreedevi Avasarala¹, Michelle Van Scoyk¹, Manoj Kumar Karuppusamy Rathinam¹, Jordi Tauler¹, Stanley Borowicz^1,2, Robert A. Winn^1,3

¹Department of Pulmonary, Critical Care, Sleep, and Allergy,University of Illinois at Chicago, ²Department of Hematology and Oncology,University of Illinois at Chicago, ³Jesse Brown Veterans Affairs Medical Center

Journal (Biology)

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Journal (Biology)

Isolating Primary Melanocyte-like Cells from the Mouse Heart

Hayoung Hwang¹, Fang Liu¹, Mark D. Levin¹, Vickas V. Patel¹

¹Penn Cardiovascular Institute, Perelman School of Medicine,University of Pennsylvania

Journal (Biology)

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Yong Wang*^1,2, En Cai*^1,2, Janet Sheung^1,2, Sang Hak Lee^1,2, Kai Wen Teng^2,3, Paul R. Selvin^1,2,3

¹Department of Physics,University of Illinois at Urbana-Champaign, ²Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, ³Center for Biophysics and Computational Biology,University of Illinois at Urbana-Champaign

Journal (Biology)

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Subarna Bhattacharya*¹, Paul W. Burridge*², Erin M. Kropp¹, Sandra L. Chuppa¹, Wai-Meng Kwok³, Joseph C. Wu², Kenneth R. Boheler^4,5, Rebekah L. Gundry^1,6

¹Department of Biochemistry,Medical College of Wisconsin, ²Stanford Cardiovascular Institute,Stanford University School of Medicine, ³Department of Anesthesiology,Medical College of Wisconsin, ⁴Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine,Hong Kong University, ⁵Division of Cardiology,Johns Hopkins University School of Medicine, ⁶Cardiovascular Research Center, Biotechnology and Bioengineering Center,Medical College of Wisconsin

Journal (Biology)

Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

Laura A. Dyer¹, Yaxu Wu¹, Cam Patterson²

¹McAllister Heart Institute,University of North Carolina at Chapel Hill, ²New York-Presbyterian Hospital/Weill-Cornell Medical Center

Journal (Biology)

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Carrie M. Spratford¹, Justin P. Kumar¹

¹Department of Biology,Indiana University

Journal (Biology)

High-throughput Titration of Luciferase-expressing Recombinant Viruses

Vanessa Garcia^1,2, Ramya Krishnan^1,2, Colin Davis^1,2, Cory Batenchuk^1,2, Fabrice Le Boeuf^1,3, Hesham Abdelbary^1,3, Jean-Simon Diallo^1,2

¹Center for Innovative Cancer Research,Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, ³Faculty of Medicine,University of Ottawa

Journal (Biology)

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Sarah Pickles¹, Nathalie Arbour², Christine Vande Velde²

¹Department of Biochemistry,Université de Montréal, CRCHUM, ²Department of Neurosciences,Université de Montréal, CRCHUM

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